Coburn S P, Mahuren J D
Anal Biochem. 1983 Mar;129(2):310-7. doi: 10.1016/0003-2697(83)90555-9.
A cation-exchange procedure for detecting nanogram quantities of all seven common vitamin B6 metabolites in a variety of biological samples is presented. The procedure uses gradient elution with a single column to effect separation of the seven compounds within 40 to 60 min, depending on the complexity of the sample. Postcolumn addition of buffer and bisulfite ion permits sensitive fluorometric detection of all B6 compounds at the same wavelengths (330 nm activation, 400 nm emission).
本文介绍了一种阳离子交换方法，用于检测各种生物样品中纳克级的所有七种常见维生素B6代谢物。该方法使用单柱梯度洗脱，根据样品的复杂程度，在40至60分钟内分离这七种化合物。柱后添加缓冲液和亚硫酸氢根离子可在相同波长（330nm激发，400nm发射）下对所有B6化合物进行灵敏的荧光检测。
