Buzoni-Gatel D, Rodolakis A
Ann Microbiol (Paris). 1983 Jan-Feb;134A(1):91-9. doi: 10.1016/0769-2609(83)90107-2.
Strains of Chlamydia psittaci isolated from faeces of a clinically healthy ewe (intestinal strain IB1) and from the foetus of an aborted ewe (abortive strain AB7) were injected into pregnant mice via the intraperitoneal route and into non-pregnant mice via the footpad route. In pregnant mice the response to inoculation was followed by recording the performance and viability of infant mice, and in the non-pregnant mice by an enlargement of the spleen and colonization of both the spleen and popliteal lymph node. The intestinal Chlamydia strain was less virulent than the abortive strain, but the actual difference of virulence depends upon the route of inoculation. Thus the intestinal strain was at least 500 times less virulent than the abortive strain and was rapidly ruled out when injected subcutaneously into the footpad, whereas it was even less than 100 times less virulent than the abortive strain, and able to induce death of some infant mice, when given intraperitoneally in pregnant mice. The mouse model of experimental subcutaneous infection via the footpad route could be used to easily test the differences of virulence between different strains of C. psittaci.
从临床健康母羊粪便中分离出的鹦鹉热衣原体菌株（肠道菌株IB1）和从流产母羊胎儿中分离出的菌株（流产菌株AB7），通过腹腔途径注射到怀孕小鼠体内，通过足垫途径注射到未怀孕小鼠体内。在怀孕小鼠中，通过记录幼鼠的表现和存活率来跟踪接种后的反应，在未怀孕小鼠中，则通过脾脏肿大以及脾脏和腘窝淋巴结的定植来跟踪。肠道衣原体菌株的毒力低于流产菌株，但实际的毒力差异取决于接种途径。因此，肠道菌株的毒力比流产菌株至少低500倍，当皮下注射到足垫时很快就被排除，而当腹腔注射到怀孕小鼠体内时，其毒力比流产菌株低不到100倍，并且能够导致一些幼鼠死亡。通过足垫途径进行实验性皮下感染的小鼠模型可用于轻松测试不同鹦鹉热衣原体菌株之间的毒力差异。
