Layachi K, Rodolakis A, Buzoni-Gatel D
INRA, Pathologie Infectieuse et Immunologie, Nouzilly, France.
Vet Res. 1993;24(1):55-65.
Specific antigens of virulent strains of Chlamydia psittaci isolated from ruminants were characterized by western blotting. The immunoblot analyses were performed with chlamydial elementary bodies from abortive (AB7 strain) or intestinal (iB1 strain) chlamydiae using mouse and rabbit immune sera raised against viable chlamydiae or proteic extracts. Eleven antigens were found to be AB7-specific, but only 4 (96, 90, 88 and 49-50 kDa) were recognized by both mouse and rabbit sera against viable AB7 strains. These could be candidates for specific diagnosis of caprine and ovine abortive chlamydiosis. No iB1-specific antigen recognized by mouse and rabbit anti-sera could be identified.
通过蛋白质印迹法对从反刍动物中分离出的鹦鹉热衣原体强毒株的特异性抗原进行了表征。使用针对活衣原体或蛋白质提取物产生的小鼠和兔免疫血清，对来自流产型（AB7株）或肠道型（iB1株）衣原体的衣原体原体进行免疫印迹分析。发现有11种抗原是AB7特异性的，但只有4种（96、90、88和49 - 50 kDa）被针对活AB7株的小鼠和兔血清所识别。这些可能是山羊和绵羊流产型衣原体病特异性诊断的候选抗原。未发现能被小鼠和兔抗血清识别的iB1特异性抗原。
