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[超薄等电聚焦在血液和分泌物痕量研究中的应用]

[The use of ultrathin isoelectric focusing in the study of traces of blood and secretions].

作者信息

Schmitter H, Kissling E

出版信息

Arch Kriminol. 1983 Jan-Feb;171(1-2):26-32.

PMID:6847324
Abstract

Four examples of the applicability of ultrathin layer isoelectric focusing in the investigation of bloodstains and stains of body secretions are given: -- differentiation of stains of human and animal blood; -- determination of fetal hemoglobin in bloodstains; -- subtyping of PGM1 isoenzymes in extracts of blood and semen stains; -- Gc typing of bloodstains using an immunofixation technique. For the visualisation of PGM1 subtypes a modified sandwich technique using a celluloseacetate membrane as separator between the focused gel and the visualisation agar is described.

摘要

给出了超薄层等电聚焦在血迹和身体分泌物污渍调查中的四个应用实例

——区分人类和动物血迹；——测定血迹中的胎儿血红蛋白；——对血液和精液污渍提取物中的PGM1同工酶进行亚型分析；——使用免疫固定技术对血迹进行Gc分型。对于PGM1亚型的可视化，描述了一种改良的夹心技术，该技术使用醋酸纤维素膜作为聚焦凝胶和可视化琼脂之间的分隔物。

相似文献

1
[The use of ultrathin isoelectric focusing in the study of traces of blood and secretions].[超薄等电聚焦在血液和分泌物痕量研究中的应用]
Arch Kriminol. 1983 Jan-Feb;171(1-2):26-32.
2
Group specific component subtyping in bloodstains by separator isoelectric focusing in micro-ultrathin polyacrylamide gels followed by immunoblotting.通过在微超薄聚丙烯酰胺凝胶中进行分离器等电聚焦，随后进行免疫印迹，对血迹中的群体特异性成分进行亚型分析。
J Forensic Sci. 1988 Sep;33(5):1267-72.
3
Subtyping of group specific component (GC) in micro-bloodstains and semen stains by isoelectric focusing in ultrathin immobilized pH gradient gels followed by enzyme immunodetection.
Electrophoresis. 1988 Aug;9(8):443-8. doi: 10.1002/elps.1150090818.
4
Subtyping phosphoglucomutase-1 in semen stains and bloodstains: a report on the method.
J Forensic Sci. 1986 Oct;31(4):1341-8.
5
Subtyping of phosphoglucomutase 1 (PGM1) in microstains of different biological materials with subsequent species differentiation without further stain material consumption.不同生物材料微量污渍中磷酸葡萄糖变位酶1（PGM1）的亚型分析及后续物种鉴别，无需消耗更多污渍材料。
Electrophoresis. 1988 Aug;9(8):438-43. doi: 10.1002/elps.1150090817.
6
A comparison of the sensitivity of typing group-specific component (Gc) and the phosphoglucomutase (PGM1) locus in control and casework bloodstains by three isoelectric focusing methods.采用三种等电聚焦方法对对照血痕和案件血痕中血型特异性成分（Gc）和磷酸葡萄糖变位酶（PGM1）位点分型的敏感性比较。
Forensic Sci Int. 1986 Oct;32(2):121-30. doi: 10.1016/0379-0738(86)90148-9.
7
[Gc subtyping in blood stains by isoelectric focusing in immobilized pH gradients].
Arch Kriminol. 1984 Jan-Feb;173(1-2):50-4.
8
Further alleles of phosphoglucomutase in human semen detected by isoelectric focusing.
J Forensic Sci. 1979 Jan;24(1):189-92.
9
Subtyping of group specific component (GC) in human semen, blood and vaginal fluid by isoelectric focusing in immobilized pH gradients.通过固定化pH梯度等电聚焦法对人精液、血液和阴道分泌物中的群体特异性成分（GC）进行亚型分析。
Electrophoresis. 1988 Sep;9(9):602-5. doi: 10.1002/elps.1150090923.
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The use of separator isoelectric focusing in micro-ultrathin polyacrylamide gels in the characterization of some polymorphic proteins of forensic science significance.在法医科学领域具有重要意义的一些多态性蛋白质的表征中，使用微超薄聚丙烯酰胺凝胶中的等电聚焦分离器。
J Forensic Sci. 1987 Nov;32(6):1558-64.

引用本文的文献

1
[A new criminologic procedure: study of L-cystine-aminopeptidase activity for the detection of seminal stains].
Z Rechtsmed. 1987;98(4):255-62. doi: 10.1007/BF00201232.
2
Comparative studies between counterimmunoelectrophoresis and microprecipitation method of identification of human minute bloodstains.对流免疫电泳法与微量沉淀法鉴定人体微量血迹的比较研究
Z Rechtsmed. 1988;99(4):227-33. doi: 10.1007/BF00204432.