Schmitter H, Kissling E
Arch Kriminol. 1983 Jan-Feb;171(1-2):26-32.
Four examples of the applicability of ultrathin layer isoelectric focusing in the investigation of bloodstains and stains of body secretions are given: -- differentiation of stains of human and animal blood; -- determination of fetal hemoglobin in bloodstains; -- subtyping of PGM1 isoenzymes in extracts of blood and semen stains; -- Gc typing of bloodstains using an immunofixation technique. For the visualisation of PGM1 subtypes a modified sandwich technique using a celluloseacetate membrane as separator between the focused gel and the visualisation agar is described.
——区分人类和动物血迹;——测定血迹中的胎儿血红蛋白;——对血液和精液污渍提取物中的PGM1同工酶进行亚型分析;——使用免疫固定技术对血迹进行Gc分型。对于PGM1亚型的可视化,描述了一种改良的夹心技术,该技术使用醋酸纤维素膜作为聚焦凝胶和可视化琼脂之间的分隔物。