Tajima M, Tokiwa K, Katsura S
Department of Legal Medicine, School of Medicine, Iwate Medical University, Morioka, Japan.
Z Rechtsmed. 1988;99(4):227-33. doi: 10.1007/BF00204432.
A minute bloodstain on a thread 2 mm in length was tested to identify human origin by counterimmunoelectrophoresis (CIE), modified CIE, and microprecipitation method (MPM), using anti-human HbA serum. The detection limits expressed as the highest dilution of human blood on the thread in positive reaction were 1:160, 1:320, and 1:320 in CIE, modified CIE, and MPM, respectively. About 1 h was required to obtain the results. In CIE and modified CIE, the detection limits diminished according to the reduction of the samples from one half to one-eighth of the thread, but not in MPM.
用抗人血红蛋白A（HbA）血清，通过对流免疫电泳（CIE）、改良CIE和微量沉淀法（MPM）对一根2毫米长的线上的微小血迹进行人类来源鉴定。以线上人血阳性反应的最高稀释倍数表示的检测限，在CIE、改良CIE和MPM中分别为1:160、1:320和1:320。获得结果大约需要1小时。在CIE和改良CIE中，随着样本从线的二分之一减少到八分之一，检测限降低，但在MPM中并非如此。
