两种花螺原体和蜜蜂螺原体的营养需求。
Nutritional requirements of two flower spiroplasmas and honeybee spiroplasma.
作者信息
Chang C J, Chen T A
出版信息
J Bacteriol. 1983 Jan;153(1):452-7. doi: 10.1128/jb.153.1.452-457.1983.
A chemically defined medium (CC-494) was used to study the nutritional requirements of three spiroplasmas representing three distinct serogroups: flower spiroplasmas [Spiroplasma floricola and FS (SR-3)] and honeybee spiroplasma [HBS (AS-576)]. Glucose, fructose, and mannose were utilized by all three spiroplasmas. In addition, the honeybee spiroplasma could ferment trehalose, FS (SR-3) could ferment sucrose, and S. floricola could ferment trehalose, sucrose, and raffinose. The three spiroplasmas varied greatly in their requirements of amino acids for growth. S. floricola was the only strain that utilized arginine. HBS (AS-576) required at least one purine and one pyrimidine base (either free base or ribonucleoside) for growth, while both flower spiroplasmas grew with only one base in the medium. Oleic acid, cholesterol, and bovine serum albumin were essential to all three spiroplasmas. Palmitic acid, which was nonessential, promoted growth significantly.
使用一种化学成分明确的培养基（CC - 494）来研究代表三个不同血清群的三种螺旋体的营养需求：花卉螺旋体[弗洛里科拉螺旋体和FS（SR - 3）]以及蜜蜂螺旋体[HBS（AS - 576）]。所有三种螺旋体都能利用葡萄糖、果糖和甘露糖。此外，蜜蜂螺旋体能发酵海藻糖，FS（SR - 3）能发酵蔗糖，弗洛里科拉螺旋体能发酵海藻糖、蔗糖和棉子糖。这三种螺旋体在生长所需的氨基酸方面差异很大。弗洛里科拉螺旋体是唯一能利用精氨酸的菌株。HBS（AS - 576）生长需要至少一种嘌呤和一种嘧啶碱基（游离碱基或核糖核苷），而两种花卉螺旋体在培养基中仅有一种碱基就能生长。油酸、胆固醇和牛血清白蛋白对所有三种螺旋体都是必需的。非必需的棕榈酸能显著促进生长。
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