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莱氏无胆甾原体的限定培养基。

DEFINED MEDIUM FOR MYCOPLASMA LAIDLAWII.

作者信息

TOURTELLOTTE M E, MOROWITZ H J, KASIMER P

出版信息

J Bacteriol. 1964 Jul;88(1):11-5. doi: 10.1128/jb.88.1.11-15.1964.

DOI:10.1128/jb.88.1.11-15.1964
PMID:14197875
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC277248/
Abstract

Tourtellotte, Mark E. (University of Connecticut, Storrs), Harold J. Morowitz, and Phil Kasimer. Defined medium for Mycoplasma laidlawii. J. Bacteriol. 88:11-15. 1964.-A defined medium for the pleuropneumonia-like organism Mycoplasma laidlawii B is described in which absolute requirements for coenzyme A and longchain fatty acids were demonstrated. This organism did not require cholesterol or macromolecules of high molecular weight, but did show a growth requirement for peptides. Optimal growth in the basal medium was obtained in the presence of two purified peptides from crystalline ribonuclease, one of which has the amino acid sequence threonine - threonine - glutamine - alanine - asparagine-lysine, and the other lysine-glutamic acid-threonine-alanine-alanine-alanine-lysine. Continuous, but suboptimal, growth was obtained with the single ribonuclease peptide: lysine-glutamic acid-threonine-alanine-alanine-alanine-lysine.

摘要

图特洛特，马克·E.（康涅狄格大学斯托尔斯分校）、哈罗德·J.莫罗维茨和菲尔·卡西默。莱氏无胆甾原体的限定培养基。《细菌学杂志》88:11 - 15。1964年。——描述了一种用于类胸膜肺炎微生物莱氏无胆甾原体B的限定培养基，其中证明了对辅酶A和长链脂肪酸的绝对需求。该微生物不需要胆固醇或高分子量的大分子，但对肽有生长需求。在基础培养基中，当存在来自结晶核糖核酸酶的两种纯化肽时可实现最佳生长，其中一种肽的氨基酸序列为苏氨酸 - 苏氨酸 - 谷氨酰胺 - 丙氨酸 - 天冬酰胺 - 赖氨酸，另一种为赖氨酸 - 谷氨酸 - 苏氨酸 - 丙氨酸 - 丙氨酸 - 丙氨酸 - 赖氨酸。使用单一的核糖核酸酶肽：赖氨酸 - 谷氨酸 - 苏氨酸 - 丙氨酸 - 丙氨酸 - 丙氨酸 - 赖氨酸可实现持续但非最佳的生长。

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