Bowman C L, Tedeschi H
Biochim Biophys Acta. 1983 Jun 10;731(2):261-6. doi: 10.1016/0005-2736(83)90017-2.
The fluorescent dye Lucifer yellow CH was microinjected electrophoretically into giant mitochondria isolated from mice maintained on a diet containing cuprizone. The dye was retained by the mitochondria, indicating that it was contained in a space bounded by a selectively permeable membrane. The labelling was reversible by reversing the polarity of the current. A study of the disappearance of the fluorescence indicates that the permeability of the mitochondrial membrane to the dye (probably the lithium and/or the potassium salts) ranges from 10(-7) to 10(-8) cm/s.
将荧光染料路西弗黄CH通过电泳微注射到从喂食含铜螯合剂饮食的小鼠中分离出的巨型线粒体中。线粒体保留了该染料，这表明它存在于由选择性渗透膜界定的空间内。通过反转电流极性，标记是可逆的。对荧光消失的研究表明，线粒体膜对该染料（可能是锂盐和/或钾盐）的渗透率范围为10^(-7)至10^(-8)厘米/秒。
