Tai T, Eisinger M, Ogata S, Lloyd K O
Cancer Res. 1983 Jun;43(6):2773-9.
Human malignant melanoma cell lines have been divided into three broad groups on the basis of morphology, pigmentation, tyrosinase levels, the 2-dimensional electrophoretic patterns of their [3H]glucosamine-labeled glycoproteins and the presence or absence of an extracellular matrix of fibronectin. The most pigmented cell lines were characterized by the synthesis of a novel glycoprotein with a molecular weight of 75,000 and the absence of a fibronectin matrix. As cultured skin melanocytes also had these characteristics, this group of melanomas appears to be the most differentiated. Melanoma cell lines in the amelanotic group were characterized by the synthesis of high levels of HLA-DR antigen and by the production of an extracellular fibronectin matrix.
人类恶性黑色素瘤细胞系已根据形态学、色素沉着、酪氨酸酶水平、其[3H]葡糖胺标记糖蛋白的二维电泳图谱以及纤连蛋白细胞外基质的有无分为三大类。色素沉着最深的细胞系的特征是合成一种分子量为75,000的新型糖蛋白且缺乏纤连蛋白基质。由于培养的皮肤黑素细胞也具有这些特征，所以这一组黑色素瘤似乎是分化程度最高的。无色素组的黑色素瘤细胞系的特征是高水平合成HLA-DR抗原并产生细胞外纤连蛋白基质。
