Olivereau M, Olivereau J
Cell Tissue Res. 1983;229(2):243-52. doi: 10.1007/BF00214973.
Prolactin (PRL) cell activity was investigated in eels kept in fresh water (FW), deionized water (DW) supplemented or not with Ca (2 mM), in Ca-enriched FW (10 mM), in normal (Ca 3.4 mM) or Ca-free 1/3 sea water (SW), and in SW (Ca 10.2 mM) or Ca-free SW (Ca 0.15 mM). Light-microscopic studies, including measurement of the nuclear area and cell height, showed that PRL cell activity, reduced in DW, is not affected by Ca supplementation. Activity is reduced in Ca-enriched FW, in 1/3 SW and in SW, conditions inducing an increase in the plasma sodium level. The lack of calcium in saline environments partly suppresses the nuclear atrophy occurring in SW. There is no significant correlation between external or total plasma calcium concentration and PRL cell activity. In artificial Ca-free SW, eels show a rapid increase in plasma osmolarity and sodium levels; there is a significant negative correlation between these two plasma values and the nuclear area or cell height of PRL cells. As in some other teleosts, plasma osmolarity and plasma sodium seem to play a more important role than external or internal calcium in controlling PRL secretion. This correlation is not apparent in eels kept in SW, having unstimulated PRL cells but active calcium-sensitive (Ca-s) cells in the pars intermedia.
研究了置于淡水（FW）、添加或不添加钙（2 mM）的去离子水（DW）、富含钙的淡水（10 mM）、正常（钙3.4 mM）或无钙的1/3海水（SW）以及海水（钙10.2 mM）或无钙海水（钙0.15 mM）中的鳗鱼催乳素（PRL）细胞活性。包括测量核面积和细胞高度在内的光学显微镜研究表明，DW中降低的PRL细胞活性不受钙补充的影响。在富含钙的FW、1/3 SW和SW中，活性降低，这些条件会导致血浆钠水平升高。盐环境中钙的缺乏部分抑制了SW中发生的核萎缩。外部或总血浆钙浓度与PRL细胞活性之间无显著相关性。在人工无钙SW中，鳗鱼的血浆渗透压和钠水平迅速升高；这两个血浆值与PRL细胞的核面积或细胞高度之间存在显著负相关。与其他一些硬骨鱼一样，在控制PRL分泌方面，血浆渗透压和血浆钠似乎比外部或内部钙发挥更重要的作用。在置于SW中的鳗鱼中，这种相关性不明显，其PRL细胞未受刺激，但中间部有活跃的钙敏感（Ca-s）细胞。
