Masurel N, de Boer G F, Anker W J, Huffels A D
Comp Immunol Microbiol Infect Dis. 1983;6(2):141-9. doi: 10.1016/0147-9571(83)90005-x.
In the period December 1979-May 1980 a respiratory disease spread rapidly through pig herds in The Netherlands. Surveillance of 12 pig farms resulted in isolation of 22 influenza A-Swine-H1N1 (Hsw1N1) strains from 9 pig herds. The morbidity rate was high but the mortality rate was nil. Retardation in growth was observed. Sera collected from affected pig herds showed a fourfold increase in haemagglutination inhibition (HI) titre against A-Swine-H1N1 virus. Sera collected on five farms showed a geometric mean HI titre against the A-H3N2 virus above 100. A significant HI titre increase against this virus was found in sera collected on three farms. These findings indicated a recent infection by this virus. A-H3N2 virus was not isolated. The Dutch Swine-1980 isolates showed in the cross-HI test a distant antigenic relationship with the classical A/Swine/Iowa/30 (H1N1) virus and one-sided close antigenic relationship with A/New Jersey/76 (H1N1) virus. HI antibody to A/Swine/Nederland/80 (H1N1) virus was found in 4, 0, and 44%, to A/New Jersey/76 (H1N1) virus in 0.5, 0.4, and 42%, and to A/Swine/Iowa/30 (H1N1) virus in 0.5, 1, and 30% of pig sera collected in 1976, 1977, and 1980, respectively. HI antibody to A/Hong Kong/68 (H3N2) virus was detected in 36, 56, and 68%, and to A/Victoria/75 (H3N2) virus in 38, 73, and 68% of these sera, respectively. The results of this study indicate that pigs in The Netherlands, like those in North America, Southeast Asia, Japan, and Western Europe harbour A-Swine-H1N1 and A-H3N2 influenza viruses and are thus potential reservoirs for future human pandemics.
1979年12月至1980年5月期间，一种呼吸道疾病在荷兰的猪群中迅速传播。对12个猪场进行监测，从9个猪群中分离出22株甲型流感病毒猪H1N1（Hsw1N1）毒株。发病率很高，但死亡率为零。观察到生长发育迟缓。从受影响猪群采集的血清显示，针对甲型流感病毒猪H1N1的血凝抑制（HI）效价增加了四倍。在五个猪场采集的血清显示，针对甲型H3N2病毒的几何平均HI效价高于100。在三个猪场采集的血清中发现针对该病毒的HI效价显著增加。这些发现表明最近感染了这种病毒。未分离出甲型H3N2病毒。荷兰1980年的猪分离株在交叉HI试验中显示与经典的甲型流感病毒猪爱荷华/30（H1N1）有较远的抗原关系，与甲型流感病毒新泽西/76（H1N1）有单向密切的抗原关系。在1976年、1977年和1980年采集的猪血清中，分别有4%、0%和44%的血清对甲型流感病毒猪荷兰/80（H1N1）有HI抗体，有0.5%、0.4%和42%的血清对甲型流感病毒新泽西/76（H1N1）有HI抗体，有0.5%、1%和30%的血清对甲型流感病毒猪爱荷华/30（H1N1）有HI抗体。分别有36%、56%和68%的这些血清检测到针对甲型流感病毒香港/68（H3N2）的HI抗体，有38%、73%和68%的这些血清检测到针对甲型流感病毒维多利亚/75（H3N2）的HI抗体。这项研究的结果表明，荷兰的猪与北美、东南亚、日本和西欧的猪一样，携带甲型流感病毒猪H1N1和甲型H3N2流感病毒，因此是未来人类大流行的潜在宿主。
