Bassüner R, Huth A, Manteuffel R, Rapoport T A
Eur J Biochem. 1983 Jun 15;133(2):321-6. doi: 10.1111/j.1432-1033.1983.tb07465.x.
Xenopus oocytes injected with poly(A)-containing RNA from developing cotyledons of field beans (Vicia faba L. var. minor) synthesize precursor polypeptides to the major storage globulins legumin and vicilin. These polypeptides are secreted into the medium without proteolytic cleavage of the legumin propolypeptides into the mature disulfide-linked alpha and beta chains. Similarly, storage globulin polypeptides from pea (Pisum sativum L.) and french bean (Phaseolus vulgaris L.) were secreted from oocytes. Inhibition of glycosylation by tunicamycin does not prevent secretion. This first report on the secretion of plant polypeptides by Xenopus oocytes shows that (a) intracellular deposition of storage proteins in membrane-bounded organelles (protein bodies) of plants and extracellular secretion have step(s) in common, and (b) the cell, in addition to the mRNA, determines the final destination of these polypeptides.
用来自蚕豆（小粒种蚕豆变种）发育中的子叶的含多聚腺苷酸的RNA注射非洲爪蟾卵母细胞，能合成主要贮藏球蛋白豆球蛋白和豌豆球蛋白的前体多肽。这些多肽被分泌到培养基中，豆球蛋白前体多肽没有被蛋白酶切割成成熟的、通过二硫键连接的α链和β链。同样，来自豌豆和菜豆的贮藏球蛋白多肽也从卵母细胞中分泌出来。衣霉素对糖基化的抑制作用并不妨碍分泌。这篇关于非洲爪蟾卵母细胞分泌植物多肽的首次报道表明：（a）植物中贮藏蛋白在膜结合细胞器（蛋白体）中的细胞内沉积和细胞外分泌有共同步骤；（b）除了mRNA外，细胞决定了这些多肽的最终去向。
