含有小鼠卫星DNA的染色质中的组蛋白乙酰化

Histone acetylation in chromatin containing mouse satellite DNA.

作者信息

Pashev I G, Dimitrov S I, Ivanov I G, Markov G G

出版信息

Eur J Biochem. 1983 Jun 15;133(2):379-82. doi: 10.1111/j.1432-1033.1983.tb07473.x.

DOI:10.1111/j.1432-1033.1983.tb07473.x

PMID:6852048

Abstract

Histone acetylation in transcriptionally inactive chromatin has been studied with chromatin containing mouse satellite DNA. The latter was obtained by digestion of nuclei from Ehrlich ascites tumor cells with the restriction nuclease Bsp, which degrades main-band DNA but leaves satellite DNA intact. The enzyme-resistant material was separated by gel filtration. Satellite DNA amounted to 65% of the total DNA in this fraction. When the cells were grown in the presence of sodium n-butyrate to inhibit histone deacetylation, a few, if any, hyperacetylated forms of core histones were found in satellite chromatin. Conversely, the highest quantity of tetraacetylated H4 molecules was found in the fractions containing the most extensively degraded chromatin.

摘要

利用含有小鼠卫星DNA的染色质对转录不活跃染色质中的组蛋白乙酰化进行了研究。后者是通过用限制性核酸酶Bsp消化艾氏腹水瘤细胞核获得的，该酶可降解主带DNA，但使卫星DNA保持完整。通过凝胶过滤分离出抗酶材料。该部分中卫星DNA占总DNA的65%。当细胞在丁酸钠存在下生长以抑制组蛋白去乙酰化时，在卫星染色质中发现了一些（如果有的话）核心组蛋白的超乙酰化形式。相反，在含有降解最广泛的染色质的部分中发现了最高量的四乙酰化H4分子。