Laurent U B, Granath K A
Exp Eye Res. 1983 Apr;36(4):481-92. doi: 10.1016/0014-4835(83)90042-8.
The molecular weight distribution of hyaluronate in the aqueous humour and vitreous body of rabbit and cattle has been determined by gel chromatography. The eluate from the column was monitored by a radioassay, whereby the molecular weight distribution of 15-20 microgram samples could be analysed. Control experiments were carried out with radioactively-labelled hyaluronate added to bovine material to estimate the degradation of the polymer during handling of the ocular fluids. It was shown that in vitro degradation does not appreciably affect the results. The analyses show a considerable polydispersity of the hyaluronate preparations. There is also a marked variation in the degree of polymerization between the two species. Hyaluronate from rabbit vitreous has a weight-average molecular weight (MW) of 2-3 X 10(6) while adult bovine vitreous displays a value of about 5-8 X 10(5). The hyaluronate of bovine vitreous varies with age. In newborn calf, a value of 3 X 10(6) was registered. This value dropped to about 5 X 10(5) in old cattle. The hyaluronate in the aqueous humour of rabbit showed a considerably higher molecular weight than that of the vitreous indicating that part of the hyaluronate in the anterior segment originates elsewhere than the vitreous. The differences between hyaluronate from the aqueous humour of adult cattle and that from the vitreous were more complex. As with the rabbit, a relatively large proportion of hyaluronate in the aqueous humour was of high-molecular weight, but, in contrast, the aqueous humour also contained material which had a lower degree of polymerization than the hyaluronate in the vitreous. The proportion of high-molecular weight material in bovine aqueous humour seemed to be lower in the summer than in the winter.
已通过凝胶色谱法测定了兔和牛眼房水及玻璃体中透明质酸的分子量分布。通过放射性测定法监测柱洗脱液，由此可分析15 - 20微克样品的分子量分布。用添加到牛材料中的放射性标记透明质酸进行对照实验，以估计眼液处理过程中聚合物的降解情况。结果表明，体外降解对结果没有明显影响。分析表明透明质酸制剂具有相当大的多分散性。两种动物之间的聚合度也有显著差异。兔玻璃体中的透明质酸重均分子量（MW）为2 - 3×10⁶，而成年牛玻璃体的该值约为5 - 8×10⁵。牛玻璃体中的透明质酸随年龄变化。新生小牛的该值为3×10⁶。在老牛中该值降至约5×10⁵。兔眼房水中的透明质酸分子量明显高于玻璃体中的，这表明前段中的部分透明质酸并非源自玻璃体。成年牛眼房水和玻璃体中透明质酸的差异更为复杂。与兔一样，眼房水中相对较大比例的透明质酸为高分子量，但相比之下，眼房水中还含有聚合度低于玻璃体中透明质酸的物质。夏季牛眼房水中高分子量物质的比例似乎低于冬季。
