Bachrach U, Abu-Elheiga L, Schnur L F
Exp Parasitol. 1983 Jun;55(3):280-8. doi: 10.1016/0014-4894(83)90024-3.
The polyamine content of the skin of BALB/c and C3H mice was determined at intervals, after injecting Leishmania tropica major. In BALB/c mice, putrescine and spermidine levels increased three- to seven-fold; in C3H mice, spontaneous recovery occurred after 3 weeks, accompanied by a reduction in putrescine and spermidine levels. Ornithine decarboxylase activity was negligible in normal, uninfected skin of both BALB/c and C3H mice, but increased steadily during infection. Treatment with drugs that inhibit the growth of leishmanial amastigotes in the skin of mice also reduced polyamine levels and ornithine decarboxylase activity of previously infected skin. There was a close correlation between the therapeutic activity of the drugs and their effect on polyamine content and synthesis. The aminoglycoside paromomycin, which was chemotherapeutically more effective than pentamidine, also had a greater effect on polyamine levels. S-adenosyl-L-Methionine decarboxylase activity in the skin of BALB/c and C3H mice was only slightly affected by the parasites. Polyamine levels and ornithine decarboxylase activity could possibly serve as means for measuring the growth of leishmanial parasites in skin and other tissues and as a measure of the efficacy of anti-leishmanial chemotherapeutics.
在注射大型热带利什曼原虫后，定期测定BALB/c和C3H小鼠皮肤中的多胺含量。在BALB/c小鼠中，腐胺和亚精胺水平增加了三到七倍；在C3H小鼠中，3周后出现自发恢复，同时腐胺和亚精胺水平降低。在正常、未感染的BALB/c和C3H小鼠皮肤中，鸟氨酸脱羧酶活性可忽略不计，但在感染期间稳步增加。用抑制小鼠皮肤中利什曼原虫无鞭毛体生长的药物进行治疗，也会降低先前感染皮肤中的多胺水平和鸟氨酸脱羧酶活性。药物的治疗活性与其对多胺含量和合成的影响之间存在密切相关性。氨基糖苷类巴龙霉素在化疗上比喷他脒更有效，对多胺水平的影响也更大。BALB/c和C3H小鼠皮肤中的S-腺苷-L-甲硫氨酸脱羧酶活性仅受到寄生虫的轻微影响。多胺水平和鸟氨酸脱羧酶活性可能作为测量利什曼原虫在皮肤和其他组织中生长的手段，以及作为抗利什曼化疗药物疗效的一种衡量标准。
