Bachrach U, Abu-Elheiga L, Talmi M, Schnur L F, El-On J, Greenblatt C L
Med Biol. 1981 Dec;59(5-6):441-7.
The relation between the grown of leishmanial parasites and polyamine biosynthesis was studied. Polyamines, mainly putrescine and spermidine, accumulated in macrophages infected with Leishmania tropica major promastigotes grown in vitro. Similar results were obtained, when tissues of BALB/C mice infected with L. tropica major were examined. A consistent increase in cellular putrescine and spermidine levels was observed in infected skin and spleen. With the accumulation of putrescine, a concomitant increase in ornithine decarboxylase activity was detected in growing leishmanial promastigotes and in macrophages supporting the growth of leishmanial amastigotes. An increase in the activity of ornithine decarboxylase was also observed in Leishmania-infected skin and spleen from BALB/C mice.
研究了利什曼原虫寄生虫生长与多胺生物合成之间的关系。多胺，主要是腐胺和亚精胺，在体外培养的感染热带利什曼原虫前鞭毛体的巨噬细胞中积累。当检查感染热带利什曼原虫的BALB/C小鼠组织时，也得到了类似的结果。在感染的皮肤和脾脏中观察到细胞内腐胺和亚精胺水平持续增加。随着腐胺的积累，在生长的利什曼原虫前鞭毛体和支持利什曼原虫无鞭毛体生长的巨噬细胞中检测到鸟氨酸脱羧酶活性随之增加。在感染利什曼原虫的BALB/C小鼠的皮肤和脾脏中也观察到鸟氨酸脱羧酶活性增加。
