菜豆叶绿体DNA物理图谱的构建及核糖体RNA和转移RNA基因的定位。

Construction of the physical map of the chloroplast DNA of Phaseolus vulgaris and localization of ribosomal and transfer RNA genes.

作者信息

Mubumbila M, Gordon K H, Crouse E J, Burkard G, Weil J H

出版信息

Gene. 1983 Mar;21(3):257-66. doi: 10.1016/0378-1119(83)90009-4.

DOI:10.1016/0378-1119(83)90009-4

Abstract

Construction of a physical map of the chloroplast DNA from Phaseolus vulgaris showed that this circular molecule is segmentally organized into four regions. Unlike other chloroplast DNAs which have analogous organization, two single-copy regions that separate two inverted repeats have been demonstrated to exist in both relative orientations, giving rise to two populations of DNA molecules. Hybridization studies using individual rRNA and tRNA species revealed the location of a set of rRNA genes and at least seven tRNA genes in each inverted repeat region, a minimum of 17 tRNA genes in the large single-copy region and one tRNA gene in the small single-copy region. The tRNA genes code for 24 tRNA species corresponding to 16 amino acids. Comparison of this gene map with those of other chloroplast DNAs suggests that DNA sequence rearrangements, involving some tRNA genes, have occurred.

摘要

菜豆叶绿体DNA物理图谱的构建表明，这种环状分子被分段组织成四个区域。与其他具有类似组织方式的叶绿体DNA不同，分隔两个反向重复序列的两个单拷贝区域已被证明以两种相对方向存在，从而产生了两种DNA分子群体。使用单个rRNA和tRNA种类进行的杂交研究揭示了一组rRNA基因的位置，每个反向重复区域至少有七个tRNA基因，大单拷贝区域至少有17个tRNA基因，小单拷贝区域有一个tRNA基因。这些tRNA基因编码对应于16种氨基酸的24种tRNA种类。将该基因图谱与其他叶绿体DNA的图谱进行比较表明，涉及一些tRNA基因的DNA序列重排已经发生。