Department of Genetics, The Royal Veterinary and Agricultural University, Bülowsvej 13, DK-1870, Copenhagen V, Denmark.
Plant Mol Biol. 1986 Mar;7(2):143-9. doi: 10.1007/BF00040140.
Isolated chloroplasts from Pisum sativum were found to contain at least 32 tRNA species. Hybridization of in vitro labeled, identified, chloroplast tRNAs to Pisum chloroplast DNA fragments revealed the locations of the tRNA genes on the circular chloroplast genome. Comparison of this gene map to the maps of Vicia faba and Phaseolus vulgaris showed that the chloroplast genomes of Pisum and Phaseolus are otherwise more closely related than either genome is to the chloroplast genome of Vicia. Furthermore, the results suggest how possible recombination events could be involved in the evolution of these three closely related, but divergent, chloroplast genomes.
从豌豆中分离出的叶绿体至少含有 32 种 tRNA。体外标记、鉴定的叶绿体 tRNA 与豌豆叶绿体 DNA 片段的杂交揭示了 tRNA 基因在圆形叶绿体基因组上的位置。将这个基因图谱与蚕豆和菜豆的图谱进行比较表明，豌豆和菜豆的叶绿体基因组比蚕豆的叶绿体基因组与它们更为接近。此外，这些结果表明，在这三个密切相关但又有差异的叶绿体基因组的进化过程中，可能涉及到重组事件。
