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合成脱氧寡核苷酸作为叶绿体转移RNA基因的通用探针。

Synthetic deoxyoligonucleotides as general probes for chloroplast transfer RNA genes.

作者信息

Nickoloff J A, Hallick R B

出版信息

Nucleic Acids Res. 1982 Dec 20;10(24):8191-210. doi: 10.1093/nar/10.24.8191.

DOI:10.1093/nar/10.24.8191
PMID:6298712
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327079/
Abstract

The utility of chemically synthesized deoxyoligonucleotides as hybridization probes for the detection of tRNA genes has been examined. Chloroplast tRNA genes were chosen for this study. Deoxyoligonucleotides complementary to highly conserved regions of chloroplast tRNA genes of both higher plants and Euglena gracilis were chemically synthesized. These synthetic probes have been used to detect tRNA genes by Southern hybridizations to restriction fragments of chloroplast DNAs. This new method of tRNA gene mapping and the oligonucleotides synthesized may be of general application to many chloroplast genomes. This is illustrated by the detection of known and unknown tRNA genes of Euglena gracilis and spinach, and unknown tRNA genes of maize and cucumber chloroplast DNAs. The precise locus and polarity of the Euglena gracilis chloroplast tRNAPhe gene has been determined. We also describe experiments which relate to the effects of the time of hybridization, the stringency of washing, and of base pair mismatches on the hybridization signal.

摘要

已对化学合成的脱氧寡核苷酸作为检测tRNA基因的杂交探针的效用进行了研究。本研究选择了叶绿体tRNA基因。化学合成了与高等植物和纤细裸藻叶绿体tRNA基因高度保守区域互补的脱氧寡核苷酸。这些合成探针已用于通过与叶绿体DNA的限制性片段进行Southern杂交来检测tRNA基因。这种新的tRNA基因定位方法以及合成的寡核苷酸可能普遍适用于许多叶绿体基因组。纤细裸藻和菠菜已知和未知tRNA基因以及玉米和黄瓜叶绿体DNA未知tRNA基因的检测证明了这一点。已确定了纤细裸藻叶绿体tRNAPhe基因的精确位置和极性。我们还描述了与杂交时间、洗涤严谨性以及碱基对错配对杂交信号的影响相关的实验。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/306e/327079/9db761859a85/nar00393-0309-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/306e/327079/ff4f54e6d353/nar00393-0300-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/306e/327079/4750db592b5a/nar00393-0303-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/306e/327079/0fb4b3778f3a/nar00393-0304-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/306e/327079/26caa165b928/nar00393-0305-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/306e/327079/dc5cd4311543/nar00393-0306-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/306e/327079/df8ba6b2a161/nar00393-0307-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/306e/327079/9db761859a85/nar00393-0309-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/306e/327079/ff4f54e6d353/nar00393-0300-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/306e/327079/da1f03dcc4f6/nar00393-0300-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/306e/327079/4750db592b5a/nar00393-0303-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/306e/327079/0fb4b3778f3a/nar00393-0304-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/306e/327079/26caa165b928/nar00393-0305-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/306e/327079/dc5cd4311543/nar00393-0306-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/306e/327079/df8ba6b2a161/nar00393-0307-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/306e/327079/9db761859a85/nar00393-0309-a.jpg

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引用本文的文献

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Characterization of the TrnD, TrnK, PsaA locus of Euglena gracilis chloroplast DNA.

本文引用的文献

1
Transcriptional origin of Euglena chloroplast tRNAs.眼虫叶绿体tRNA的转录起源
Proc Natl Acad Sci U S A. 1976 Jun;73(6):1984-8. doi: 10.1073/pnas.73.6.1984.
2
The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA.合成寡核苷酸作为杂交探针的应用。II. 混合序列寡核苷酸与兔β-珠蛋白DNA的杂交
Nucleic Acids Res. 1981 Feb 25;9(4):879-94. doi: 10.1093/nar/9.4.879.
3
Compilation of sequences of tRNA genes.转运RNA基因序列的汇编。
秀丽隐杆藻叶绿体 DNA 的 TrnD、TrnK、PsaA 基因座的特征。
Plant Mol Biol. 1987 Jul;8(4):327-36. doi: 10.1007/BF00021312.
4
Efficient repair of all types of single-base mismatches in recombination intermediates in Chinese hamster ovary cells. Competition between long-patch and G-T glycosylase-mediated repair of G-T mismatches.中国仓鼠卵巢细胞中重组中间体各类单碱基错配的高效修复。长片段修复与G-T糖基化酶介导的G-T错配修复之间的竞争。
Genetics. 1998 Aug;149(4):1935-43. doi: 10.1093/genetics/149.4.1935.
Nucleic Acids Res. 1982 Jan 22;10(2):r57-81.
4
Compilation of tRNA sequences.转运RNA序列的汇编。
Nucleic Acids Res. 1982 Jan 22;10(2):r1-55.
5
Specific detection of human and rabbit glucagon mRNA using a synthetic oligodeoxynucleotide.
Biochem Biophys Res Commun. 1980 Apr 14;93(3):941-7. doi: 10.1016/0006-291x(80)91166-3.
6
Location of a phenylalanine tRNA gene on the physical map of the Euglena gracilis chloroplast genome.苯丙氨酸tRNA基因在纤细裸藻叶绿体基因组物理图谱上的定位。
Gene. 1982 Mar;17(3):337-9. doi: 10.1016/0378-1119(82)90150-0.
7
Sequencing of 16S--23S spacer in a ribosomal RNA operon of Euglena gracilis chloroplast DNA reveals two tRNA genes.纤细裸藻叶绿体DNA核糖体RNA操纵子中16S - 23S间隔区的测序揭示了两个tRNA基因。
Nature. 1980 Aug 28;286(5776):908-10. doi: 10.1038/286908a0.
8
Selective in vitro transcription of Euglena chloroplast ribosomal RNA genes by a transcriptionally active chromosome.通过转录活性染色体对眼虫叶绿体核糖体RNA基因进行选择性体外转录。
J Biol Chem. 1980 Apr 25;255(8):3786-92.
9
Euglena gracilis chloroplast transfer RNA transcription units. II. Nucleotide sequence analysis of a tRNAVal-tRNAAsn-tRNAArg-tRNALeu gene cluster.纤细裸藻叶绿体转移RNA转录单位。II. 一个缬氨酸转运RNA-天冬酰胺转运RNA-精氨酸转运RNA-亮氨酸转运RNA基因簇的核苷酸序列分析
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10
Euglena gracilis chloroplast transfer RNA transcription units. I. Physical map of the transfer RNA gene loci.纤细裸藻叶绿体转移RNA转录单位。I. 转移RNA基因位点的物理图谱。
J Biol Chem. 1982 Mar 25;257(6):3258-64.