Waxman F J, Coe J E
Immunobiology. 1983 Mar;164(2):171-83. doi: 10.1016/S0171-2985(83)80008-4.
The proliferative response of spleen cells, obtained from Syrian hamsters sensitized to hen egg albumin emulsified in complete Freund's adjuvant, is lower in magnitude than the response of draining lymph node cells. In this study, the cellular regulatory mechanisms which may lead to splenic hyporesponsiveness were examined. Although unfractionated spleen cells were not suppressive, the addition of nylon wool nonadherent normal spleen cells to sensitized draining lymph node (target) cells markedly suppressed antigen- but not mitogen-induced proliferation. Suppressor cell activity was not detected in normal lymph nodes. Suppression could be overcome by culturing splenic suppressor plus target cell mixtures in the presence of large quantities of antigen. Suppressor cell activity was radioresistant. In addition to nonadherent suppressor cells, the hamster spleen also contains an adherent cell population(s) which amplified antigen-induced proliferation. Adherent cells with amplifying activity were also present in lymph nodes. The addition of adherent cells abrogated splenic suppression of proliferation. Collectively, these data indicate that the hamster spleen contains both suppressive and amplifying leukocyte subpopulations which may be involved in the regulation of the immune response to certain antigenic stimuli.
从用完全弗氏佐剂乳化的鸡卵白蛋白致敏的叙利亚仓鼠获得的脾细胞增殖反应，其强度低于引流淋巴结细胞的反应。在本研究中，对可能导致脾低反应性的细胞调节机制进行了研究。虽然未分离的脾细胞没有抑制作用，但将尼龙毛非黏附正常脾细胞添加到致敏的引流淋巴结（靶）细胞中，可显著抑制抗原诱导而非丝裂原诱导的增殖。在正常淋巴结中未检测到抑制细胞活性。通过在大量抗原存在的情况下培养脾抑制细胞加靶细胞混合物，可克服抑制作用。抑制细胞活性具有抗放射性。除了非黏附抑制细胞外，仓鼠脾脏还含有一个（或多个）黏附细胞群体，可增强抗原诱导的增殖。具有增强活性的黏附细胞也存在于淋巴结中。添加黏附细胞可消除脾脏对增殖的抑制作用。总体而言，这些数据表明，仓鼠脾脏含有抑制性和增强性白细胞亚群，它们可能参与对某些抗原刺激的免疫反应调节。
