Shek P N
J Immunol. 1977 Jul;119(1):83-90.
Rabbits were immunized in the footpad with diphtheria toxoid in complete Freund's adjuvant. At various times after immunization, cells harvested from the spleen, the draining (immune) and the opposing (control) lymph nodes (LN) were assayed for their proliferative response to incorporate tritiated thymidine upon exposure to the priming antigen. Although the immune LN cells responded by a substantial incorporation of thymidine, cells from the control LN and the spleen either did not respond or responded feebly. An enhancement in the response of the immune LN cells was observed when they were cultured in the presence of nonresponsive spleen cells. Pretreatment of spleen cells with mitomycin C did not abolish the enhancement. This suggests that the target cells which respond to the antigen are derived from the immune LN whereas the promoter cells which enhance the response are present in the spleen. The removal of adhering cells by glass wool columns and of Ig-bearing cells by anti-Ig immunoabsorbent columns from the spleen did not reduce the enhancing capacity of the nonadhering cells. Conversely, the killing of splenic T cells by specific heterologous antiserum directed against rabbit thymus lymphocyte antigen abolished the enhancement. Thus, the promoter cell which facilitates the enhancement has been characterized as a nonadherent, splenic T cell.
用完全弗氏佐剂中的白喉类毒素对家兔进行足垫免疫。免疫后的不同时间，对从脾脏、引流（免疫）和对侧（对照）淋巴结（LN）收获的细胞进行检测，以观察其在接触致敏抗原后掺入氚标记胸腺嘧啶核苷的增殖反应。尽管免疫淋巴结细胞通过大量掺入胸腺嘧啶核苷做出反应，但对照淋巴结和脾脏的细胞要么没有反应，要么反应微弱。当免疫淋巴结细胞在无反应性的脾细胞存在下培养时，观察到其反应增强。用丝裂霉素C预处理脾细胞并没有消除这种增强作用。这表明对抗原做出反应的靶细胞来源于免疫淋巴结，而增强反应的促进细胞存在于脾脏中。用玻璃棉柱去除脾脏中的黏附细胞以及用抗Ig免疫吸附柱去除含Ig细胞，并没有降低非黏附细胞的增强能力。相反，用针对兔胸腺淋巴细胞抗原的特异性异源抗血清杀死脾脏T细胞则消除了这种增强作用。因此，促进这种增强作用的促进细胞已被鉴定为非黏附性的脾脏T细胞。
