de Baetselier P, Vaeck M, de Smet W, Ron Y
Immunology. 1980 Dec;41(4):997-1003.
An in vitro assay which measures specific antigen-induced proliferation of primed rabbit lymph node and peripheral blood cells is described. This response was found to be mediated by T cells, since it could be obtained with nylon-wool passed cells and cells which do not adhere to anti-Ig-coated plastic plates. The proliferative response was found to be highly antigen-specific and restricted to the draining lymph node if assessed up to 15 days post-priming. Purified T cells required antigen-pulsed accessory cells to proliferate. The proliferative response of peripheral blood lymphocytes was found to be low unless the lymphocytes were fractionated on insolubilized histamine. The histamine-adherent cells could suppress the above peripheral blood response, implying a certain regulatory role of suppressive cells in the periphery. The ease, reproducibility and specificity of the assay provides a simple method to evaluate the characteristics of a T-cell response in the rabbit.
本文描述了一种体外检测方法，该方法可测量经致敏的兔淋巴结和外周血细胞对特定抗原的增殖反应。发现这种反应是由T细胞介导的，因为使用尼龙毛柱过滤后的细胞以及不粘附于抗Ig包被塑料板的细胞也能产生这种反应。如果在致敏后15天内进行评估，发现增殖反应具有高度抗原特异性，且局限于引流淋巴结。纯化的T细胞需要经抗原刺激的辅助细胞才能增殖。发现外周血淋巴细胞的增殖反应较低，除非淋巴细胞在不溶性组胺上进行分离。组胺粘附细胞可抑制上述外周血反应，这表明抑制性细胞在外周具有一定的调节作用。该检测方法的简便性、可重复性和特异性为评估兔T细胞反应特性提供了一种简单方法。
