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被伯纳特立克次氏体持续感染的小鼠巨噬细胞样细胞系的溶酶体反应。

Lysosomal response of a murine macrophage-like cell line persistently infected with Coxiella burnetii.

作者信息

Akporiaye E T, Rowatt J D, Aragon A A, Baca O G

出版信息

Infect Immun. 1983 Jun;40(3):1155-62. doi: 10.1128/iai.40.3.1155-1162.1983.

DOI:10.1128/iai.40.3.1155-1162.1983
PMID:6852916
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC348171/
Abstract

The lysosomal response of a murine macrophage-like tumor cell line (J774) during persistent infection with Coxiella burnettii was examined. By using acid phosphatase as a lysosomal marker, it was shown that phagosome-lysosome fusion occurred in J774 cells persistently infected with C. burnetii. This observation was verified using thorium dioxide, an electron-dense compound that is sequestered in secondary lysosomes. The phagolysosomes contained viable replicating rickettsiae. Spectrofluorometric analysis indicated that the phagolysosomal pH of persistently infected cells was acidic. In attempts to correlate rickettsial survival with lysosome function, the activities of several lysosomal enzymes were assayed in both infected and uninfected cells. Activities of acid phosphatase and beta-acetylglucosaminidase were not significantly altered during infection. However, infected cells appeared to display slightly higher intracellular lysozyme, beta-glucuronidase, and beta-galactosidase activities.

摘要

研究了鼠巨噬细胞样肿瘤细胞系（J774）在持续感染伯纳特柯克斯体期间的溶酶体反应。通过使用酸性磷酸酶作为溶酶体标记物，发现吞噬体-溶酶体融合发生在持续感染伯纳特柯克斯体的J774细胞中。使用二氧化钍（一种电子致密化合物，被隔离在次级溶酶体中）验证了这一观察结果。吞噬溶酶体中含有存活的正在复制的立克次体。荧光分光光度分析表明，持续感染细胞的吞噬溶酶体pH呈酸性。为了将立克次体的存活与溶酶体功能相关联，在感染和未感染的细胞中均检测了几种溶酶体酶的活性。感染期间酸性磷酸酶和β-乙酰氨基葡萄糖苷酶的活性没有显著改变。然而，感染细胞似乎表现出略高的细胞内溶菌酶、β-葡萄糖醛酸酶和β-半乳糖苷酶活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ce8/348171/c3630bf20f45/iai00141-0320-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ce8/348171/3fb1160138af/iai00141-0317-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ce8/348171/65fb94452e41/iai00141-0318-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ce8/348171/0fd7485b103a/iai00141-0319-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ce8/348171/c3630bf20f45/iai00141-0320-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ce8/348171/3fb1160138af/iai00141-0317-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ce8/348171/65fb94452e41/iai00141-0318-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ce8/348171/0fd7485b103a/iai00141-0319-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ce8/348171/c3630bf20f45/iai00141-0320-a.jpg

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