Oreste U, Calligaris V, Ruffilli A
Int Arch Allergy Appl Immunol. 1983;71(3):267-70. doi: 10.1159/000233401.
34 sera of patients allergic to the pollen of Parietaria officinalis were analyzed by ultracentrifugation on sucrose gradient (40-10%) in 0.9% NaCl at 24,000 rpm with a SW 27.1 rotor for complexed circulating IgE. Total and specific anti-PO IgE content of the gradient's fractions were determined by radioimmunoassay. IgE presence was demonstrated in at least two peaks; one corresponding to monomeric IgE, another corresponding to IgE with a sedimentation constant higher than that of the monomers. The ratio between monomeric and complexed IgE was different for each serum. Discordant results of PRIST and RIST analyses suggest that complexed IgE has fewer sites available for reaction with anti-IgE than the monomers.
采用SW 27.1转头，在24,000转/分钟的条件下，于0.9%氯化钠溶液中、40%-10%的蔗糖梯度上对34份对药用墙草花粉过敏患者的血清进行超速离心，以分析其中的循环IgE复合物。通过放射免疫分析法测定梯度组分中的总抗PO IgE和特异性抗PO IgE含量。在至少两个峰中检测到IgE的存在；一个峰对应单体IgE，另一个峰对应沉降常数高于单体的IgE。每份血清中单体IgE与复合IgE的比例各不相同。PRIST和RIST分析结果不一致，提示复合IgE与抗IgE反应的位点比单体少。
