Lindberg R E, Pinnas J L, Jones J F
J Allergy Clin Immunol. 1982 Apr;69(4):388-96. doi: 10.1016/0091-6749(82)90151-8.
The interaction between pollen grains, polymorphonuclear leukocytes (PMNs) and serum results in the formation of "pollen rosettes" and the production of chemiluminescence (CL). Significantly more CL is produced by cells exposed ot pollen and sera from nonallergic individuals compared with sera from allergic individuals. The source of the PMNs, however, has no effect on the CL produced, When IgG is isolated from the sera of allergic individuals and added to the test system, a large CL response is seen that is inhibited by the IgG-depleted fraction. No similar inhibition is produced by the IgG-depleted fraction of sera from nonallergic individuals. The inhibitor of CL in sera from allergic individuals appears to be IgE, since its removal leads to an enhanced CL response. These results suggest that competition for a limited number of binding sites on pollen grains exists between pollen specific IgG and IgE antibodies in serum from allergic patients.
花粉粒、多形核白细胞（PMN）与血清之间的相互作用导致“花粉花环”的形成以及化学发光（CL）的产生。与过敏个体的血清相比，非过敏个体的花粉和血清所接触的细胞产生的CL明显更多。然而，PMN的来源对产生的CL没有影响。当从过敏个体的血清中分离出IgG并添加到测试系统中时，会观察到强烈的CL反应，该反应被IgG缺失部分所抑制。非过敏个体血清的IgG缺失部分不会产生类似的抑制作用。过敏个体血清中CL的抑制剂似乎是IgE，因为去除它会导致CL反应增强。这些结果表明，过敏患者血清中的花粉特异性IgG和IgE抗体之间存在对花粉粒上有限数量结合位点的竞争。
