Weiskopf R B, Townsley M I, Riordan K K, Harris D, Chadwick K
J Appl Physiol Respir Environ Exerc Physiol. 1983 Apr;54(4):978-83. doi: 10.1152/jappl.1983.54.4.978.
To construct acid-base curves and alignment nomograms for swine blood, we added known amounts of acid or base to aliquots of swine blood with three different concentrations of hemoglobin. Pairs of blood samples were equilibrated at two different CO2 partial pressures, and blood pH was measured. Data were analyzed by computer, and mean values were created for complete data of four swine. Curve and alignment nomograms were constructed by computer. The resultant nomograms for swine blood differ from those constructed by Siggaard-Andersen for human blood, most importantly at base-excess values of +10 to +25 mmol/l. The possible reasons for the observed differences are discussed and, although not completely resolved, may be related in large measure to the accuracy of pH determination and the methodology of nomogram construction.
为构建猪血液的酸碱曲线和校准列线图，我们向三种不同血红蛋白浓度的猪血液等分试样中添加已知量的酸或碱。将成对的血液样本在两种不同的二氧化碳分压下进行平衡，并测量血液pH值。通过计算机对数据进行分析，并为四只猪的完整数据创建平均值。通过计算机构建曲线和校准列线图。所得的猪血液列线图与西格gaard - 安德森为人类血液构建的列线图不同，最重要的是在碱剩余值为+10至+25 mmol/l时。讨论了观察到的差异的可能原因，尽管尚未完全解决，但可能在很大程度上与pH测定的准确性和列线图构建方法有关。
