卵清蛋白和扁白蛋白单晶的初步X射线分析。

Preliminary x-ray analysis of single crystals of ovalbumin and plakalbumin.

作者信息

Miller M, Weinstein J N, Wlodawer A

出版信息

J Biol Chem. 1983 May 10;258(9):5864-6.

Abstract

Two crystal forms of hen ovalbumin have been grown and were characterized by x-ray diffraction. Crystals of ovalbumin grown at pH 5.4 are monoclinic, belonging to the space group C2 (a = 116.5 A, b = 41.8 A, c = 70.5 A, beta = 101.5 degrees). At pH 5.8 or higher ovalbumin forms triclinic crystals of space group P1 (a = 62.0 A, b = 84.3 A, c = 72.2 A, alpha = 87.4 degrees, beta = 103.9 degrees, gamma = 108.7 degrees). These crystals are usually small and difficult to grow reproducibly. Crystals of plakalbumin (a proteolytic modification of ovalbumin) are much easier to grow and appear suitable for three-dimensional structure determination. Their space group is C2 (a = 99.9 A, b = 102.0 A, c = 79.3 A, beta = 112.1 degrees) with two plakalbumin molecules in each asymmetric unit.

摘要

已培养出两种鸡卵清蛋白晶体形式，并通过X射线衍射对其进行了表征。在pH 5.4条件下生长的卵清蛋白晶体为单斜晶系，属于空间群C2（a = 116.5 Å，b = 41.8 Å，c = 70.5 Å，β = 101.5°）。在pH 5.8或更高时，卵清蛋白形成空间群为P1的三斜晶系晶体（a = 62.0 Å，b = 84.3 Å，c = 72.2 Å，α = 87.4°，β = 103.9°，γ = 108.7°）。这些晶体通常较小且难以重复生长。副白蛋白（卵清蛋白的一种蛋白水解修饰产物）晶体更容易生长，似乎适合用于三维结构测定。其空间群为C2（a = 99.9 Å，b = 102.0 Å，c = 79.3 Å，β = 112.1°），每个不对称单元中有两个副白蛋白分子。