Lukács G L, Nagy I, Lustyik G, Balázs G
J Cancer Res Clin Oncol. 1983;105(3):280-4. doi: 10.1007/BF00395759.
Parallel studies were performed using microfluorimetric DNA determination and X-ray microanalysis on the same thyroid biopsy material to compare the intranuclear DNA and monovalent electrolyte contents (Na+, K+, Cl-). Samples were taken from apparently healthy, adenomatous, and cancerous parts of human thyroid glands removed surgically. The time interval was less than 1 min. The tissues were classified by the pathologist into four main classes: 1) Normal thyroid tissue; 2) benign adenomas; 3) differentiated (follicular and papillary) carcinomas; and 4) anaplastic cancers. The results revealed that the level of aneuploidization showed an increase parallel with the malignancy of the studied tumor. At the same time, a similar tendency was found in the average values of the intranuclear Na+:K+ ratios. The results obtained in this way confirm the possibility that the electric properties of the cell membrane, that is the sustained membrane depolarization, may have a role in the regulation of DNA synthesis and in mitogenesis. These results may offer new diagnostic and/or therapeutic possibilities.
使用微量荧光法测定DNA和X射线微量分析对相同的甲状腺活检材料进行平行研究，以比较细胞核内DNA和单价电解质含量（Na +、K +、Cl -）。样本取自手术切除的人类甲状腺明显健康、腺瘤性和癌性部分。时间间隔小于1分钟。病理学家将组织分为四个主要类别：1）正常甲状腺组织；2）良性腺瘤；3）分化型（滤泡状和乳头状）癌；4）间变性癌。结果显示，非整倍体化水平与所研究肿瘤的恶性程度呈平行增加。同时，在细胞核内Na +:K +比值的平均值中也发现了类似趋势。以这种方式获得的结果证实了细胞膜的电特性，即持续的膜去极化，可能在DNA合成调节和有丝分裂发生中起作用的可能性。这些结果可能提供新的诊断和/或治疗可能性。
