Nagy I, Tóth L, Szállási Z, Lampé I
J Cancer Res Clin Oncol. 1987;113(2):197-202. doi: 10.1007/BF00391444.
Energy-dispersive X-ray microanalysis was performed on human biopsy materials taken during laryngoscopic intervention in 18 cases. The removed tissue pieces were divided into two parts. One of them was used for pathohistological studies, the other was processed for X-ray microanalysis by the freeze-fracture freeze-drying method. Of the cases investigated 4 proved to be of benign character, whereas the rest contained carcinoma planocellulare keratoides or non-keratoides. Bulk specimen energy-dispersive X-ray microanalysis of 135 cells from the benign tissue samples revealed an average Na+/K+ molar ratio of 0.13 +/- 0.01 (SEM) in the intracellular space, with a regular Gaussian distribution. In the cases of carcinomas 641 cells were measured, the average of the same ratio was 0.67 +/- 0.03 (SEM) due mostly to an increase in the Na+ content. The distribution of data was apparently not normal in the cancerous samples. These observations and some theoretical considerations support the notion that the intracellular Na+/K+ ratio correlates with the proliferative capacity of tissues. The relevance of some recent biochemical results is also discussed in this respect.
对18例喉镜干预期间获取的人体活检材料进行了能量色散X射线微量分析。切除的组织块分为两部分。其中一部分用于病理组织学研究，另一部分通过冷冻断裂冷冻干燥法进行X射线微量分析。在所研究的病例中，4例为良性，其余病例含有角化性扁平细胞癌或非角化性癌。对良性组织样本中的135个细胞进行大量样本能量色散X射线微量分析，结果显示细胞内空间的平均Na+/K+摩尔比为0.13±0.01（标准误），呈规则的高斯分布。在癌症病例中，测量了641个细胞，由于Na+含量增加，同一比例的平均值为0.67±0.03（标准误）。癌性样本中的数据分布明显不正常。这些观察结果和一些理论思考支持了细胞内Na+/K+比值与组织增殖能力相关的观点。在这方面还讨论了一些近期生化结果的相关性。
