Fong M H, Abbiati A, Benfenati E, Caccia S
J Chromatogr. 1983 Mar 25;259(1):141-9. doi: 10.1016/s0021-9673(01)87987-5.
A simple and rapid high-performance liquid chromatographic method is described for the quantitative analysis of the psychotropic drug minaprine and three of its metabolites (M1, M3 and M11), including one as yet undetected metabolite (M11) known as a monoamine oxidase type A inhibitor in vitro. After selective extraction all four compounds were separated on a reversed-phase muBondapak C18 column using sodium acetate (0.03 M)-acetonitrile-methanol (88:7:5) (pH 3.3) as the mobile phase. The eluted compounds were detected with a UV detector at 254 nm. The sensitivity of the method is 0.02 microgram per millilitre of body fluid or per gram of tissue for M1 and M11 and 0.05 microgram per minaprine and M3. The method has been applied successfully to the determination of minaprine and the metabolites in plasma and brain and is compared here with an gas-liquid chromatographic method with an electron-capture detector previously developed for the detection of minaprine and M11. M11 was identified in rat urine by gas chromatography-mass spectrometry.
本文描述了一种简单快速的高效液相色谱法，用于定量分析精神药物米那普明及其三种代谢物（M1、M3和M11），其中包括一种尚未检测到的代谢物（M11），该代谢物在体外被称为A型单胺氧化酶抑制剂。经过选择性萃取后，所有四种化合物在反相μBondapak C18柱上进行分离，流动相为醋酸钠（0.03 M）-乙腈-甲醇（88:7:5）（pH 3.3）。洗脱的化合物用紫外检测器在254 nm处进行检测。该方法对M1和M11的灵敏度为每毫升体液或每克组织0.02微克，对米那普明和M3的灵敏度为0.05微克。该方法已成功应用于血浆和脑组织中米那普明及其代谢物的测定，并在此与先前开发的用于检测米那普明和M11的带电子捕获检测器的气液色谱法进行比较。通过气相色谱-质谱法在大鼠尿液中鉴定出了M11。
