Macchi G, Bentivoglio M, Minciacchi D, Molinari M
J Comp Neurol. 1983 Apr 1;215(2):121-134. doi: 10.1002/cne.902150202.
The topographical interrelations of claustroneocortical cells and their degree of divergent collateralization were investigated in cat by means of retrograde fluorescent double labeling. The tracers Fast Blue (FB) and Nuclear Yellow (NY) were injected in several combinations in two different cortical fields. FB-labeled and NY-labeled cells were found in all cases in the insular portion of the ipsilateral claustrum (CII). The distributions of labeled cells confirmed the organization reported in previous studies. In some cases the two labeled cell populations were segregated in different parts of the CII; in other cases there was more or less overlap in the two distributions. The degree of overlap was not simply related to the topographic proximity of the injected cortical territories. In all cases the vast majority of cells were single labeled. In one case both FB and NY were injected in the same cortical area in order to control that claustral axons can retrogradely transport two tracers back to the same parent cell bodies. In this control case CII cells were double labeled. All together these data indicate that widely divergent claustroneocortical projection take origin mainly from separate cells.
利用逆行荧光双标记法，对猫脑岛皮质细胞的局部相互关系及其分支发散程度进行了研究。将示踪剂快蓝（FB）和核黄（NY）以几种组合方式注射到两个不同的皮质区域。在所有情况下，同侧屏状核（CII）的岛叶部分均发现了FB标记和NY标记的细胞。标记细胞的分布证实了先前研究中报道的组织结构。在某些情况下，两种标记的细胞群分隔在CII的不同部位；在其他情况下，两种分布或多或少存在重叠。重叠程度与注射皮质区域的局部接近程度并无简单关联。在所有情况下，绝大多数细胞为单标记。在一个案例中，将FB和NY都注射到同一皮质区域，以验证屏状核轴突能否将两种示踪剂逆行运输回同一个亲代细胞体。在这个对照案例中，CII细胞为双标记。所有这些数据表明，广泛发散的屏状核-新皮质投射主要起源于不同的细胞。
