通过微量酶联免疫吸附测定法测量哺乳动物DNA中的紫外线诱导光损伤

Measurement of ultraviolet light-induced photolesions in mammalian DNA by microELISA.

作者信息

Leipold B, Remy W, Adelmann-Grill B

出版信息

J Immunol Methods. 1983 May 27;60(1-2):69-76. doi: 10.1016/0022-1759(83)90336-8.

Abstract

A sensitive microELISA was developed for titration of anti-UV-DNA antisera and measurement of photolesions in DNA using UV-irradiated DNA as antigen coupled to the wells of microtitre plates. Efficient and reproducible immobilization of the antigen required precoating of the plates with poly(L-lysine). Antisera from 2 rabbits immunized with UVdsDNA recognized specifically thymine dimer associated lesions. The antiserum from one animal was used to probe photodamage in DNA. Lesions induced by an irradiation dose as small as 2.5 J/m2 could be detected.

摘要

开发了一种灵敏的微量酶联免疫吸附测定法，用于滴定抗紫外线DNA抗血清，并以紫外线照射的DNA作为与微量滴定板孔偶联的抗原，来测量DNA中的光损伤。抗原的有效且可重复固定需要用聚（L-赖氨酸）预包被平板。用紫外线双链DNA免疫的2只兔子的抗血清特异性识别与胸腺嘧啶二聚体相关的损伤。用其中一只动物的抗血清探测DNA中的光损伤。可以检测到低至2.5 J/m2照射剂量诱导的损伤。