• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Detection of laser--UV microirradiation-induced DNA photolesions by immunofluorescent staining.

作者信息

Cremer C, Cremer T, Fukuda M, Nakanishi K

出版信息

Hum Genet. 1980;54(1):107-10. doi: 10.1007/BF00279058.

DOI:10.1007/BF00279058
PMID:6993338
Abstract

A low-power laser-UV microbeam of wavelength 257 nm was used for microirradiation of a small part of the nucleus of Chinese hamster cells. Following fixation in interphase or in the subsequent metaphase indirect immunofluorescent staining was performed with antiserum to photoproducts of DNA treated with far UV light. The results show that antibodies specific for UV-irradiated DNA can be used for a direct detection of laser-UV microirradiation-induced DNA photolesions. The potential usefulness of this method for investigation of the spatial arrangement of chromosomes in the interphase nucleus is discussed.

摘要

相似文献

1
Detection of laser--UV microirradiation-induced DNA photolesions by immunofluorescent staining.
Hum Genet. 1980;54(1):107-10. doi: 10.1007/BF00279058.
2
Sister chromatid exchange (SCE) induced by laser-UV-microirradiation: correlation between the distribution of photolesions and the distribution of SCEs.激光紫外线微照射诱导的姐妹染色单体交换（SCE）：光损伤分布与SCE分布之间的相关性。
Basic Life Sci. 1984;29 Pt A:181-97. doi: 10.1007/978-1-4684-4889-4_15.
3
UV micro-irradiation of the Chinese hamster cell nucleus and caffeine post-treatment. Immunocytochemical localization of DNA photolesions in cells with partial and generalized chromosome shattering.中国仓鼠细胞核的紫外线微照射及咖啡因后处理。DNA光损伤在部分和全身性染色体破碎细胞中的免疫细胞化学定位。
Mutat Res. 1983 Feb;107(2):465-76. doi: 10.1016/0027-5107(83)90184-7.
4
Immunocytochemical localization of chromatin regions UV-microirradiated in S phase or anaphase. Evidence for a territorial organization of chromosomes during cell cycle of cultured Chinese hamster cells.处于S期或后期的经紫外线微辐照染色质区域的免疫细胞化学定位。培养的中国仓鼠细胞在细胞周期中染色体存在区域化组织的证据。
Exp Cell Res. 1983 Nov;149(1):257-69. doi: 10.1016/0014-4827(83)90397-x.
5
Induction of chromosome shattering by ultraviolet light and caffeine: the influence of different distributions of photolesions.紫外线和咖啡因诱导染色体破碎：光损伤不同分布的影响
Mutat Res. 1986 Oct;163(1):33-40. doi: 10.1016/0027-5107(86)90055-2.
6
Laser UV microirradiation of interphase nuclei and post-treatment with caffeine. A new approach to establish the arrangement of interphase chromosomes.间期细胞核的激光紫外线微照射及咖啡因后处理。一种确定间期染色体排列的新方法。
Hum Genet. 1976 Dec 29;35(1):83-9. doi: 10.1007/BF00295622.
7
Laser-UV-microirradiation of Chinese hamster cells: the influence of the distribution of photolesions on unscheduled DNA synthesis.
Photochem Photobiol. 1981 Jun;33(6):925-8. doi: 10.1111/j.1751-1097.1981.tb05514.x.
8
Analysis of chromosome positions in the interphase nucleus of Chinese hamster cells by laser-UV-microirradiation experiments.通过激光紫外线微照射实验分析中国仓鼠细胞间期核中的染色体位置。
Hum Genet. 1982;62(3):201-9. doi: 10.1007/BF00333519.
9
Rabl's model of the interphase chromosome arrangement tested in Chinese hamster cells by premature chromosome condensation and laser-UV-microbeam experiments.通过早熟染色体凝聚和激光紫外线微束实验在中国仓鼠细胞中测试的拉布尔间期染色体排列模型。
Hum Genet. 1982;60(1):46-56. doi: 10.1007/BF00281263.
10
[Local unscheduled DNA synthesis following irradiation of a portion of mitotic chromosomes or interphase nucleus with UV microbeam].[用紫外线微束照射有丝分裂染色体的一部分或间期核后局部非预定DNA合成]
Tsitologiia. 1976 Sep;18(9):1111-4.

引用本文的文献

1
Cell-Type-Dependent Recruitment Dynamics of FUS Protein at Laser-Induced DNA Damage Sites.细胞类型依赖性的 FUS 蛋白在激光诱导的 DNA 损伤部位的募集动态。
Int J Mol Sci. 2024 Mar 20;25(6):3526. doi: 10.3390/ijms25063526.
2
Recent Microscopy Advances and the Applications to Huntington's Disease Research.近期显微镜技术的进展及其在亨廷顿病研究中的应用。
J Huntingtons Dis. 2022;11(3):269-280. doi: 10.3233/JHD-220536.
3
In Situ Analysis of DNA-Protein Complex Formation upon Radiation-Induced DNA Damage.在辐射诱导的 DNA 损伤下 DNA-蛋白质复合物形成的原位分析。

本文引用的文献

1
Antibodies to photoproducts of deoxyribonucleic acids irradiated with ultraviolet light.
Science. 1966 Sep 30;153(3744):1666-7. doi: 10.1126/science.153.3744.1666.
2
The rationale for an ordered arrangement of chromatin in the interphase nucleus.间期细胞核中染色质有序排列的基本原理。
Am J Hum Genet. 1968 Sep;20(5):440-60.
3
Effects of ultraviolet micro-irradiation on different parts of the cell. II. Cytological observations and unscheduled DNA synthesis after partial nuclear irradiation.紫外线微辐照对细胞不同部位的影响。II. 部分细胞核辐照后的细胞学观察和DNA的非预定合成
Int J Mol Sci. 2019 Nov 15;20(22):5736. doi: 10.3390/ijms20225736.
4
Systematic analysis of DNA damage induction and DNA repair pathway activation by continuous wave visible light laser micro-irradiation.连续波可见光激光微照射诱导DNA损伤及激活DNA修复途径的系统分析
AIMS Genet. 2017 Feb 21;4(1):47-68. doi: 10.3934/genet.2017.1.47. eCollection 2017.
5
Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells.激光微照射在哺乳动物细胞单链和双链断裂修复检测中的应用
J Vis Exp. 2017 Sep 5(127):56265. doi: 10.3791/56265.
6
Recollections of a scientific journey published in human genetics: from chromosome territories to interphase cytogenetics and comparative genome hybridization.科学之旅的回忆在人类遗传学中发表：从染色体领域到间期细胞遗传学和比较基因组杂交。
Hum Genet. 2014 Apr;133(4):403-16. doi: 10.1007/s00439-014-1425-5. Epub 2014 Feb 7.
7
DNA in motion during double-strand break repair.在双链断裂修复过程中移动的 DNA。
Trends Cell Biol. 2013 Nov;23(11):529-36. doi: 10.1016/j.tcb.2013.05.006. Epub 2013 Jul 15.
8
DNA multiphoton absorption generates localized damage for studying repair dynamics in live cells.DNA 多光子吸收可产生局部损伤，用于研究活细胞中的修复动力学。
Biophys J. 2011 Nov 2;101(9):2294-303. doi: 10.1016/j.bpj.2011.09.031. Epub 2011 Nov 1.
9
DNA damage response.DNA 损伤反应。
Cold Spring Harb Perspect Biol. 2011 Jan 1;3(1):a000745. doi: 10.1101/cshperspect.a000745.
10
A novel and simple micro-irradiation technique for creating localized DNA double-strand breaks.一种新颖而简单的微照射技术，用于产生局部 DNA 双链断裂。
Nucleic Acids Res. 2010 Jul;38(12):e129. doi: 10.1093/nar/gkq226. Epub 2010 Apr 12.
Exp Cell Res. 1971 Mar;65(1):129-39. doi: 10.1016/s0014-4827(71)80058-7.
4
DNA-degradation products from mammalian cells irradiated with ultra-violet light.
Int J Radiat Biol Relat Stud Phys Chem Med. 1969 Jul;15(5):411-24. doi: 10.1080/09553006914550701.
5
Excision repair (dimer excision, strand breakage and repair replication) in primary cultures of eukaryotic (bovine) cells.真核（牛）细胞原代培养中的切除修复（二聚体切除、链断裂和修复复制）
Exp Cell Res. 1972 Sep;74(1):67-80. doi: 10.1016/0014-4827(72)90482-x.
6
An ultraviolet laser microbeam for 257 nm.一台波长为257纳米的紫外激光微束。
Microsc Acta. 1974 Mar;75(4):331-7.
7
The internal order of the interphase nucleus.间期细胞核的内部秩序。
Humangenetik. 1974;25(4):265-97. doi: 10.1007/BF00336904.
8
The use of antinucleoside antibodies to probe the organization of chromosomes denatured by ultraviolet irradiation.使用抗核苷抗体探测经紫外线照射变性的染色体的组织结构。
Exp Cell Res. 1974 Sep;88(1):31-9. doi: 10.1016/0014-4827(74)90614-4.
9
Immunological demonstration of the disappearance of pyrimidine dimers from nuclei of cultured human cells.培养的人类细胞核中嘧啶二聚体消失的免疫学证明。
Exp Cell Res. 1972 Oct;74(2):480-6. doi: 10.1016/0014-4827(72)90404-1.
10
Effects of laser uv-microirradiation (lambda = 2573 A) on proliferation of Chinese hamster cells.激光紫外微辐射（波长=2573埃）对中国仓鼠细胞增殖的影响。
Radiat Res. 1976 Apr;66(1):106-21.