Zouali M, Stollar B D
J Immunol Methods. 1986 Jun 10;90(1):105-10. doi: 10.1016/0022-1759(86)90390-x.
Pretreatment of polystyrene microplate wells with certain doses of UV light enhances their capacity for binding to single-stranded DNA, double stranded DNA and various synthetic polynucleotides. The use of UV-irradiated plates to immobilize nucleic acid antigens provides a simple, rapid, and specific ELISA for measuring anti-nucleic acid antibodies. The assay is at least as sensitive as the more complex method of precoating plates with poly(L-lysine). It is useful for detection of anti-DNA antibodies in sera of systemic lupus erythematous patients, as well as in culture fluids of murine and human anti-DNA-secreting hybridomas.
用一定剂量的紫外线预处理聚苯乙烯微孔板孔，可增强其与单链DNA、双链DNA及各种合成多核苷酸的结合能力。使用紫外线照射过的板来固定核酸抗原，可为检测抗核酸抗体提供一种简单、快速且特异的酶联免疫吸附测定（ELISA）方法。该测定方法至少与用聚（L-赖氨酸）预包被板这种更复杂的方法一样灵敏。它可用于检测系统性红斑狼疮患者血清中的抗DNA抗体，以及鼠源和人源分泌抗DNA的杂交瘤细胞培养液中的抗DNA抗体。
