一种使用紫外线处理过的聚苯乙烯微孔板来测量核酸抗原抗体的快速酶联免疫吸附测定法。
A rapid ELISA for measurement of antibodies to nucleic acid antigens using UV-treated polystyrene microplates.
作者信息
Zouali M, Stollar B D
出版信息
J Immunol Methods. 1986 Jun 10;90(1):105-10. doi: 10.1016/0022-1759(86)90390-x.
Pretreatment of polystyrene microplate wells with certain doses of UV light enhances their capacity for binding to single-stranded DNA, double stranded DNA and various synthetic polynucleotides. The use of UV-irradiated plates to immobilize nucleic acid antigens provides a simple, rapid, and specific ELISA for measuring anti-nucleic acid antibodies. The assay is at least as sensitive as the more complex method of precoating plates with poly(L-lysine). It is useful for detection of anti-DNA antibodies in sera of systemic lupus erythematous patients, as well as in culture fluids of murine and human anti-DNA-secreting hybridomas.
用一定剂量的紫外线预处理聚苯乙烯微孔板孔，可增强其与单链DNA、双链DNA及各种合成多核苷酸的结合能力。使用紫外线照射过的板来固定核酸抗原，可为检测抗核酸抗体提供一种简单、快速且特异的酶联免疫吸附测定（ELISA）方法。该测定方法至少与用聚（L-赖氨酸）预包被板这种更复杂的方法一样灵敏。它可用于检测系统性红斑狼疮患者血清中的抗DNA抗体，以及鼠源和人源分泌抗DNA的杂交瘤细胞培养液中的抗DNA抗体。
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