Berman W J, Gil J, Jennett R B, Tuma D, Sorrell M F, Rubin E
Lab Invest. 1983 Jun;48(6):760-7.
The effects of chronic ethanol intoxication on hepatic microtubules are controversial. We, therefore, designed a morphometric study to examine ethanol-induced alterations in microtubules in vivo and in vitro. Using a computer-assisted point-counting system we determined the volume, surface, and length densities of hepatic microtubules, in addition to those of other hepatic organelles. Consistent with previous qualitative descriptions, chronic ethanol feeding caused increases in the volume and surface densities of mitochondria and microsomes. These changes account, in part, for the increased protein content of hepatocytes after chronic ethanol consumption. However, there was no effect on the volume, surface, or length densities of hepatic microtubules, nor on their calculated radii. The structure of isolated bovine brain microtubules after polymerization in vitro was not changed by ethanol or its metabolites, acetaldehyde and acetate. These data indicate that neither chronic ethanol intoxication nor the presence of ethanol, or its metabolites, affects the number or structure of microtubules.
慢性乙醇中毒对肝微管的影响存在争议。因此，我们设计了一项形态计量学研究，以检查乙醇在体内和体外诱导的微管改变。使用计算机辅助点计数系统，我们除了测定其他肝细胞器的体积、表面积和长度密度外，还测定了肝微管的这些参数。与先前的定性描述一致，长期给予乙醇导致线粒体和微粒体的体积和表面积密度增加。这些变化部分解释了长期摄入乙醇后肝细胞蛋白质含量的增加。然而，乙醇对肝微管的体积、表面积或长度密度及其计算半径均无影响。体外聚合后分离的牛脑微管结构不受乙醇及其代谢产物乙醛和乙酸盐的影响。这些数据表明，慢性乙醇中毒以及乙醇或其代谢产物的存在均不影响微管的数量或结构。
