Varga M, Buris L
College of Pharmacy, Center for Drug Design and Delivery, University of Florida, Gainesville 32610.
Exp Mol Pathol. 1990 Apr;52(2):249-57. doi: 10.1016/0014-4800(90)90010-b.
Biochemical considerations suggest that bioflavonoids may be effective antihepatotoxic and hepatoprotective agents. We, therefore, designed a morphometric study to examine the effect of (+)-cyanidanol-3 on ethanol-induced hepatocellular alterations. Using a stereologic point and intersection counting method we determined the volume fraction of cytoplasmic components, the surface area of cytoplasmic membranes, some parameters of mitochondria, and numerical densities of some organelles, after chronic ethanol intoxication and hepato-protective treatment of rats. Consistent with previous qualitative and quantitative descriptions, chronic ethanol feeding caused increases in parameters of mitochondria, microsome, and Golgi complex. The hepatoprotective (+)-cyanidanol-3 treatment restored most of the morphologic distortion caused by ethanol ingestion. The (+)-cyanidanol-3 treatment alone caused an elevation of the surface area of smooth endoplasmic reticulum and nonsignificant changes in mitochondrial parameters, possibly due to its inducing effect on microsomes and activating effect on mitochondrial enzymes. These data indicate that bioflavonoids could be of potential benefit in hepatotoxic or alcohol-related liver disease.
生化方面的考虑表明，生物类黄酮可能是有效的抗肝毒性和肝脏保护剂。因此，我们设计了一项形态计量学研究，以检验(+)-氰定醇-3对乙醇诱导的肝细胞改变的影响。我们采用体视学的点计数和交点计数方法，在大鼠经慢性乙醇中毒及肝脏保护治疗后，测定了细胞质成分的体积分数、细胞质膜的表面积、线粒体的一些参数以及某些细胞器的数量密度。与先前的定性和定量描述一致，慢性乙醇喂养导致线粒体、微粒体和高尔基体复合体的参数增加。具有肝脏保护作用的(+)-氰定醇-3治疗使大部分由乙醇摄入引起的形态学畸变得以恢复。单独使用(+)-氰定醇-3治疗导致滑面内质网表面积增加，而线粒体参数无显著变化，这可能是由于其对微粒体的诱导作用和对线粒体酶的激活作用。这些数据表明，生物类黄酮可能对肝毒性或酒精相关肝病具有潜在益处。
