Wilkinson L F, Thomson B M, Pannell L K
J Anal Toxicol. 1983 Mar-Apr;7(2):72-5. doi: 10.1093/jat/7.2.72.
A discussion of the methods reported for the analysis of orphenadrine is given. The drug levels in biological specimens and the methods reported vary widely. The stability and extractability of orphenadrine is investigated and a method suggested, which uses subtilisin digestion of tissue followed by extraction with 1-chlorobutane. Body fluids are extracted directly by 1-chlorobutane after pH adjustment. Analysis is performed by gas chromatography with a nitrogen selective detector and no interferences are observed from bland specimens. The following levels are results from a case of a 19-year-old male who was found dead 2.5 hours after last being seen alive. This laboratory analysed the case using diphenhydramine as an internal standard: blood 18.1 micrograms/mL, liver 242 micrograms/g, urine 7.0 micrograms/mL, stomach contents 1452 mg.
本文讨论了已报道的去甲麻黄碱分析方法。生物样本中的药物水平以及所报道的方法差异很大。对去甲麻黄碱的稳定性和可萃取性进行了研究，并提出了一种方法，该方法是先用枯草杆菌蛋白酶消化组织，然后用1-氯丁烷萃取。体液在调节pH值后直接用1-氯丁烷萃取。采用配有氮选择性检测器的气相色谱法进行分析，未观察到来自空白样本的干扰。以下是一名19岁男性的案例结果，该男性最后一次被看到还活着2.5小时后被发现死亡。本实验室以苯海拉明作为内标分析该案例：血液中为18.1微克/毫升，肝脏中为242微克/克，尿液中为7.0微克/毫升，胃内容物中为1452毫克。
