Danel L, Souweine G, Monier J C, Saez S
J Steroid Biochem. 1983 May;18(5):559-63. doi: 10.1016/0022-4731(83)90131-0.
The binding of estrogen in preparations of human peripheral blood mononuclear cells, as well as by splenic and thymic cells is demonstrated by three different approaches (Dextran-coated charcoal method, whole cell assay, and gel filtration on a sepharose 4B column). Scatchard's analysis of [3H]-moxestrol (R2858) and [3H]-estradiol binding proves the existence of a single class of receptor sites having a dissociation constant of 0.18-2.4 X 10(-9) M. Physicochemical properties of the binder, including binding capacity and steroid specificity, are quite similar to those reported for the thymus of small mammalian species or human thymoma.
雌激素与人外周血单核细胞制剂以及脾细胞和胸腺细胞的结合通过三种不同方法得以证实（葡聚糖包被活性炭法、全细胞测定法以及在琼脂糖4B柱上进行凝胶过滤）。对[3H]-莫昔司醇（R2858）和[3H]-雌二醇结合进行的Scatchard分析证明存在一类解离常数为0.18 - 2.4×10⁻⁹ M的单一受体位点。结合剂的物理化学性质，包括结合能力和类固醇特异性，与报道的小型哺乳动物物种的胸腺或人类胸腺瘤的性质非常相似。
