Seiki K, Imanishi Y, Haruki Y
Endocrinol Jpn. 1978 Jun;25(3):289-94. doi: 10.1507/endocrj1954.25.289.
The in vivo uptake of 3H-estradiol-17beta by the various lymphoid tissues, fat tissue, skeletal muscle and circulating blood of castrated male and female mice of C57BL strain was studied after an intravenous injection of the hormone. In both sexes the highest uptake was by the bone marrow and fat tissue, followed by the spleen, thymus and mesenteric lymph node. The lowest was by the muscle and the blood serum. The lymphoid tissues in the female took up a slightly more amount of radioactivity than those in the male until 1 hr after the injection, but thereafter the uptake was at almost the same level between both sexes. The lymphoid tissues showed a relative and long-term retention of the radioactivity, as compared with that in the muscle and blood serum. The binding of 3H-estradiol-17beta by the lymphoid tissue cytosol and their binding specificity for the hormone were examined in vitro by Sephadex G-100 column analysis. All the tissue cytosols tested contained estradiol-binding component(-s). Among those the thymic cytosol showed to contain the component(-s) which is fairly specific to this hormone. These results suggest that there is a relatively high uptake and retention of estrogen in the lymphoid tissues in which it is bound to cytoplasmic binding component(-s).
静脉注射激素后，研究了C57BL品系去势雌雄小鼠的各种淋巴组织、脂肪组织、骨骼肌和循环血液对³H-17β-雌二醇的体内摄取情况。在两性中，骨髓和脂肪组织摄取量最高，其次是脾脏、胸腺和肠系膜淋巴结。肌肉和血清摄取量最低。注射后1小时内，雌性的淋巴组织摄取的放射性略多于雄性，但此后两性的摄取量几乎处于同一水平。与肌肉和血清相比，淋巴组织显示出对放射性的相对长期保留。通过Sephadex G-100柱分析在体外检测了³H-17β-雌二醇与淋巴组织胞浆的结合及其对该激素的结合特异性。所有测试的组织胞浆都含有雌二醇结合成分。其中，胸腺胞浆显示含有对该激素相当特异的成分。这些结果表明，淋巴组织中存在相对较高的雌激素摄取和保留，雌激素在其中与细胞质结合成分结合。
