Mazzone R W, Kornblau S M
Microvasc Res. 1983 May;25(3):343-57. doi: 10.1016/0026-2862(83)90024-9.
We examined the effects of vascular and transpulmonary (Ptp) pressures on endothelial vesicles in nonedematous and edematous dog lungs by transmission electron microscopy (TEM). Edema was defined as a 30% increase in lung weight. Lungs were prepared for TEM by rapid freezing followed by freeze substitution. Using a random sampling procedure, the size and numerical density (Nv) of vesicles was obtained by standard morphometric techniques and the percentage of cytoplasm occupied by vesicles (Vv) calculated. Results show that at Ptp = 5 cm H2O, vascular pressure has no influence on the variables examined in nonedematous and edematous lungs. At Ptp = 25 cm H2O, increases in vascular pressure were associated with significant decreases in the percent cytoplasm occupied by vesicles for both series of experiments. When nonedematous lungs were compared to edematous lungs, we found increases in vesicle size, Nv, and the percentage of endothelial cell cytoplasm occupied by vesicles. The results confirm the increase in vesicles associated with edema. This result does not appear to be due to increased vascular pressure per se, although time related influences could be involved.
我们通过透射电子显微镜（TEM）研究了血管压力和跨肺压（Ptp）对非水肿和水肿犬肺内皮小泡的影响。水肿定义为肺重量增加30%。通过快速冷冻然后冷冻置换的方法制备用于TEM检查的肺组织。采用随机抽样程序，通过标准形态计量学技术获得小泡的大小和数量密度（Nv），并计算小泡所占细胞质的百分比（Vv）。结果表明，在Ptp = 5 cm H₂O时，血管压力对非水肿和水肿肺中所检测的变量没有影响。在Ptp = 25 cm H₂O时，在两个系列实验中，血管压力的升高均与小泡所占细胞质百分比的显著降低相关。当比较非水肿肺和水肿肺时，我们发现小泡大小、Nv以及内皮细胞细胞质中被小泡占据的百分比均增加。结果证实了与水肿相关的小泡增多。该结果似乎并非由于血管压力本身增加所致，尽管可能涉及与时间相关的影响。
