Pumo D E, Viceps-Madore D, Chiu J F
Mol Cell Biochem. 1983;51(2):133-9. doi: 10.1007/BF00230399.
An enzyme-linked immunosorbent assay (ELISA) was developed for identification and quantification of nuclear antigens (nonhistone protein-DNA complexes from chromatin). Until now, the complement fixation assay has been the only immunoassay routinely applied to nonhistone protein-DNA complexes. The ELISA is considerably more sensitive than the micro-complement fixation test for assaying the immunospecificity of nuclear protein-DNA complexes. Dilutions of rabbit antisera as great as 1:6400 could be used to detect nanogram quantities of antigen, chicken reticulocyte chromatin or dehistonized rat liver chromatin.
已开发出一种酶联免疫吸附测定法（ELISA），用于鉴定和定量核抗原（来自染色质的非组蛋白 - DNA复合物）。到目前为止，补体结合试验一直是唯一常规应用于非组蛋白 - DNA复合物的免疫测定法。在测定核蛋白 - DNA复合物的免疫特异性方面，ELISA比微量补体结合试验灵敏得多。高达1:6400的兔抗血清稀释液可用于检测纳克量的抗原、鸡网织红细胞染色质或去组蛋白大鼠肝染色质。
