Pumo D E, Wierzbicki R, Chiu J F
Biochemistry. 1980 May 27;19(11):2362-7. doi: 10.1021/bi00552a013.
Antibodies to chicken reticulocyte dehistonized chromatin were produced. These antibodies can distinguish between chromatin from reticulocytes and erythrocytes and have no activity with chicken liver chromatin. The antibodies will also differentiate between reticulocytes and erythrocytes by the the horseradish peroxidase bridge localization technique. The nonhistone protein components of the antigenic complex are very tightly bound to the DNA, removable by extraction with sodium dodecyl sulfate but not 2.5 M NaCl-5 M urea. Our data indicate that the antigen complexes could actually be present in both reticulocytes and erythrocytes but that chromatin condensation causes a structural masking of the complexes which coincides with the known decline in transcriptive activity of the erythrocyte.
制备了针对鸡网织红细胞去组蛋白染色质的抗体。这些抗体能够区分网织红细胞和红细胞的染色质，并且对鸡肝染色质无活性。通过辣根过氧化物酶桥定位技术，这些抗体还能区分网织红细胞和红细胞。抗原复合物的非组蛋白蛋白质成分与DNA紧密结合，可通过十二烷基硫酸钠提取去除，但不能被2.5M NaCl - 5M尿素去除。我们的数据表明，抗原复合物实际上可能同时存在于网织红细胞和红细胞中，但染色质凝聚导致复合物的结构被掩盖，这与红细胞转录活性的已知下降相一致。
