d(G-C)结构的盐浓度依赖性的1H核Overhauser效应及圆二色性研究
A 1H nOe and CD study of the salt-concentration dependence of the structure of d(G-C).
作者信息
Reid D G, Salisbury S A, Williams D H
出版信息
Nucleic Acids Res. 1983 Jun 11;11(11):3779-93. doi: 10.1093/nar/11.11.3779.
Abstract
Intra- and internucleotide 1H nuclear Overhauser effects (nOes) have been observed in the synthetic decancleoside nonaphosphate d(G-C)5 at low and high salt concentrations, and in conditions under which duplex formation is complete. Comparison of the nOes with interproton distances derived from model and X-ray structures suggest that the duplex is right-handed up to a sodium chloride concentration of 4M, in contrast to the behaviour of poly [d(G-C)]. C.D. spectra are consistent with this suggestion.
摘要
在低盐和高盐浓度下，以及在双链体形成完全的条件下，已在合成的十聚脱氧胞苷九磷酸d（G-C）5中观察到核苷酸内和核苷酸间的1H核Overhauser效应（nOes）。将这些nOes与从模型和X射线结构得出的质子间距离进行比较表明，与聚[d（G-C）]的行为相反，在氯化钠浓度高达4M时双链体是右手螺旋的。圆二色光谱与这一推测一致。
相似文献
1
A 1H nOe and CD study of the salt-concentration dependence of the structure of d(G-C).d(G-C)结构的盐浓度依赖性的1H核Overhauser效应及圆二色性研究
Nucleic Acids Res. 1983 Jun 11;11(11):3779-93. doi: 10.1093/nar/11.11.3779.
2
Use of inter-proton nuclear Overhauser effects to assign the nuclear magnetic resonance spectra of oligodeoxynucleotide and hybrid duplexes in aqueous solution.利用质子间核Overhauser效应确定水溶液中寡脱氧核苷酸和杂交双链体的核磁共振谱。
Eur J Biochem. 1983 Sep 15;135(2):307-14. doi: 10.1111/j.1432-1033.1983.tb07654.x.
3
An investigation into the solution structures of two self-complementary DNA oligomers, 5'-d(C-G-T-A-C-G) and 5'-d(A-C-G-C-G-C-G-T), by means of nuclear-Overhauser-enhancement measurements.通过核Overhauser增强测量对两种自互补DNA寡聚体5'-d（C-G-T-A-C-G）和5'-d（A-C-G-C-G-C-G-T）的溶液结构进行的研究。
Biochem J. 1984 Aug 1;221(3):723-36. doi: 10.1042/bj2210723.
4
A----Z transition in the synthetic hexanucleotide (dCdGfl)3.合成六核苷酸（dCdGfl）3中的A到Z转变
FEBS Lett. 1985 Mar 25;182(2):365-9. doi: 10.1016/0014-5793(85)80334-3.
5
Conversions of poly(2-aminodeoxyadenylate-5-halodeoxyuridylate) from B to A forms in high salt. An NMR and circular dichroism study.聚（2-氨基脱氧腺苷酸-5-卤代脱氧尿苷酸）在高盐条件下从B型到A型的转变。一项核磁共振和圆二色性研究。
Biochemistry. 1986 Nov 18;25(23):7464-70. doi: 10.1021/bi00371a031.
6
Proton nuclear Overhauser effect study of the structure of a deoxyoligonucleotide duplex in aqueous solution.水溶液中脱氧寡核苷酸双链体结构的质子核Overhauser效应研究。
Biochemistry. 1983 Apr 12;22(8):2019-25. doi: 10.1021/bi00277a044.
7
Antibiotic-DNA interactions: intermolecular nuclear Overhauser effects in the netropsin-d(C-G-C-G-A-A-T-T-C-G-C-G) complex in solution.抗生素与DNA的相互作用：溶液中鱼精蛋白-d（C-G-C-G-A-A-T-T-C-G-C-G）复合物中的分子间核Overhauser效应
Proc Natl Acad Sci U S A. 1982 Nov;79(21):6424-8. doi: 10.1073/pnas.79.21.6424.
8
Conformational analysis of d(C3G3), a B-family duplex in solution.溶液中B族双链体d(C3G3)的构象分析。
Biochemistry. 1989 Mar 21;28(6):2452-9. doi: 10.1021/bi00432a016.
9
Probing the three-dimensional structures of DNA and RNA oligonucleotides in solution by nuclear Overhauser enhancement measurements.通过核Overhauser效应测量探究溶液中DNA和RNA寡核苷酸的三维结构。
FEBS Lett. 1985 Jan 7;179(2):187-98. doi: 10.1016/0014-5793(85)80516-0.
10
The B----Z transition in two synthetic oligonucleotides: d(C-2-amino-ACGTG) and d(m5CGCAm5CGTGCG) studied by IR, NMR and CD spectroscopies.通过红外光谱、核磁共振光谱和圆二色光谱研究的两种合成寡核苷酸：d(C-2-氨基-ACGTG) 和 d(m5CGCAm5CGTGCG) 中的B-Z转变
Nucleic Acids Res. 1984 Aug 10;12(15):6291-305. doi: 10.1093/nar/12.15.6291.
引用本文的文献
1
DNA CTG triplet repeats involved in dynamic mutations of neurologically related gene sequences form stable duplexes.参与神经相关基因序列动态突变的DNA CTG三联体重复序列形成稳定的双链体。
Nucleic Acids Res. 1995 Nov 11;23(21):4303-11. doi: 10.1093/nar/23.21.4303.
2
Transcriptional enhancer related DNA sequences: anomalous 1H NMR NOE crosspeaks.转录增强子相关DNA序列：异常的1H NMR NOE交叉峰。
Nucleic Acids Res. 1992 Feb 11;20(3):525-32. doi: 10.1093/nar/20.3.525.
本文引用的文献
1
Correlations between deoxyribonucleic acid structural parameters and calculated circular dichroism spectra.脱氧核糖核酸结构参数与计算所得圆二色光谱之间的相关性。
Biochemistry. 1981 Jan 6;20(1):73-8. doi: 10.1021/bi00504a013.
2
Left-handed double helical DNA: variations in the backbone conformation.左手双螺旋DNA：主链构象的变化
Science. 1981 Jan 9;211(4478):171-6. doi: 10.1126/science.7444458.
3
Crystal structure analysis of a complete turn of B-DNA.B型DNA完整一圈的晶体结构分析。
Nature. 1980 Oct 23;287(5784):755-8. doi: 10.1038/287755a0.
4
High-salt d(CpGpCpG), a left-handed Z' DNA double helix.高盐条件下的d（CpGpCpG），一种左手Z'型DNA双螺旋结构。
Nature. 1980 Aug 7;286(5773):567-73. doi: 10.1038/286567a0.
5
Changes in the helical repeat of poly(dG-m5dC) . poly(dG-m5dC) and poly(dG-dC) . poly(dG-dC) associated with the B-Z transition.与B-Z转变相关的聚（dG-m5dC）·聚（dG-m5dC）和聚（dG-dC）·聚（dG-dC）螺旋重复序列的变化。
Nature. 1981;293(5829):233-5. doi: 10.1038/293233a0.
6
Characterization of alternating deoxyribonucleic acid conformations in solution by phosphorus-31 nuclear magnetic resonance spectroscopy.用磷-31核磁共振光谱法表征溶液中交替脱氧核糖核酸构象
Biochemistry. 1981 May 26;20(11):3049-55. doi: 10.1021/bi00514a010.
7
Left-handed deoxyribonucleic acid double helix in solution.溶液中的左手螺旋脱氧核糖核酸双螺旋
Biochemistry. 1981 Mar 31;20(7):2036-41. doi: 10.1021/bi00510a046.
8
The anatomy of A-, B-, and Z-DNA.A-DNA、B-DNA和Z-DNA的结构
Science. 1982 Apr 30;216(4545):475-85. doi: 10.1126/science.7071593.
9
Right-handed and left-handed DNA: studies of B- and Z-DNA by using proton nuclear Overhauser effect and P NMR.右手螺旋和左手螺旋DNA：利用质子核Overhauser效应和磷核磁共振对B型和Z型DNA的研究
Proc Natl Acad Sci U S A. 1982 Mar;79(5):1413-7. doi: 10.1073/pnas.79.5.1413.
10
Z-DNA: vacuum ultraviolet circular dichroism.Z型DNA：真空紫外圆二色性
Proc Natl Acad Sci U S A. 1981 Aug;78(8):4801-4. doi: 10.1073/pnas.78.8.4801.
Zotero 插件