通过核Overhauser增强测量对两种自互补DNA寡聚体5'-d（C-G-T-A-C-G）和5'-d（A-C-G-C-G-C-G-T）的溶液结构进行的研究。
An investigation into the solution structures of two self-complementary DNA oligomers, 5'-d(C-G-T-A-C-G) and 5'-d(A-C-G-C-G-C-G-T), by means of nuclear-Overhauser-enhancement measurements.
作者信息
Gronenborn A M, Clore G M, Kimber B J
出版信息
Biochem J. 1984 Aug 1;221(3):723-36. doi: 10.1042/bj2210723.
Abstract
A 500 MHz 1H-n.m.r. study on two self-complementary alternating pyrimidine-purine oligodeoxyribonucleotides, 5'-d(C-G-T-A-C-G) and 5'-d(A-C-G-C-G-C-G-T), is presented. By using the proton-proton nuclear Overhauser effect virtually complete assignments are obtained and a large number of interproton distances [113 in the case of 5'-d(C-G-T-A-C-G) and 79 in the case of 5'-d(A-C-G-C-G-C-G-T)], both intra- and inter-nucleotide, are determined. The interproton-distance data are consistent with an overall right-handed B-DNA-type structure for both oligonucleotides, in agreement with their B-type c.d. spectra. However, whereas 5'-d(C-G-T-A-C-G) adopts a conventional B-type structure with a mononucleotide repeating unit, the interproton-distance data provide evidence that 5'-d(A-C-G-C-G-C-G-T) has a dinucleotide repeating unit consisting of alternation in glycosidic bond and sugar pucker conformations.
摘要
本文介绍了对两个自我互补的交替嘧啶 - 嘌呤寡聚脱氧核糖核苷酸5'-d(C-G-T-A-C-G)和5'-d(A-C-G-C-G-C-G-T)进行的500兆赫¹H - 核磁共振研究。通过使用质子 - 质子核Overhauser效应，几乎完成了所有的归属，并确定了大量的质子间距离（5'-d(C-G-T-A-C-G)的情况为113个，5'-d(A-C-G-C-G-C-G-T)的情况为79个），包括核苷酸内和核苷酸间的距离。质子间距离数据与两种寡核苷酸的整体右手B - DNA型结构一致，这与它们的B型圆二色光谱相符。然而，虽然5'-d(C-G-T-A-C-G)采用具有单核苷酸重复单元的传统B型结构，但质子间距离数据表明5'-d(A-C-G-C-G-C-G-T)具有由糖苷键和糖环构象交替组成的二核苷酸重复单元。
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