Funk C D, Powell W S
Prostaglandins. 1983 Feb;25(2):299-309. doi: 10.1016/0090-6980(83)90112-0.
Particulate fractions from fetal calf aorta convert 8,11,14-eicosatrienoic acid to a number of products derived from 12-hydroperoxy-8,10-heptadecadienoic acid, including 2 stereoisomers of 11,12-epoxy-10-hydroxy-8-heptadecenoic acid (11,12e-10h-17:1), which were identified by mass spectrometry. In the early stages of the reaction, considerable amounts of the epoxyhydroxy isomers were formed, but the amounts of these products decreased as the reaction continued. There was a concomitant increase in the formation of 10,11,12-trihydroxy-8-heptadecenoic acid (10,11,12th-17:1), which was present only in small amounts initially. Incubation of the 2 isomers of 11,12e-10h-17:1 with microsomal and cytosolic fractions resulted in their conversion to isomers of 10,11,12th-17:1. No hydrolysis of the epoxides occurred in the presence of boiled tissue fractions.
胎牛主动脉的微粒部分将8,11,14-二十碳三烯酸转化为多种源自12-氢过氧-8,10-十七碳二烯酸的产物，包括11,12-环氧-10-羟基-8-十七碳烯酸（11,12e-10h-17:1）的2种立体异构体，这些异构体通过质谱法得以鉴定。在反应的早期阶段，形成了大量的环氧羟基异构体，但随着反应的持续，这些产物的量减少。同时，10,11,12-三羟基-8-十七碳烯酸（10,11,12th-17:1）的形成有所增加，该产物最初仅少量存在。将11,12e-10h-17:1的2种异构体与微粒体和胞质部分一起孵育，导致它们转化为10,11,12th-17:1的异构体。在煮沸的组织部分存在的情况下，环氧化物未发生水解。
