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贡氏疟原虫的体外培养

The in vitro cultivation of Plasmodium gonderi.

作者信息

Guo S C, Chin W, Collins W E

出版信息

Am J Trop Med Hyg. 1983 May;32(3):473-4. doi: 10.4269/ajtmh.1983.32.473.

DOI:10.4269/ajtmh.1983.32.473
PMID:6859396
Abstract

Plasmodium gonderi, one of the vivax-type simian parasites, has been cultured in vitro by the candle jar method and by the sealed flask method. It showed a 48-hour asexual cycle and maintained its morphologic integrity. The effect of different sera (human, rabbit, and horse) was tested in culture and compared with results using rhesus serum. Growth was lower in rabbit and horse serum, but higher in human serum. Compared with individual human and rhesus serum, the multiplication rate in combined rhesus and human sera (1:1) was greater. In addition, human erythrocytes proved refractory to invasion by P. gonderi in vitro.

摘要

冈氏疟原虫是间日疟类型的猿寄生虫之一,已通过烛缸法和密封培养瓶法进行体外培养。它表现出48小时的无性繁殖周期,并保持其形态完整性。在培养中测试了不同血清(人、兔和马血清)的效果,并与使用恒河猴血清的结果进行了比较。在兔血清和马血清中生长较慢,但在人血清中生长较快。与单独的人血清和恒河猴血清相比,恒河猴血清与人血清按1:1混合后的增殖率更高。此外,体外实验证明人红细胞对冈氏疟原虫的入侵具有抗性。

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The in vitro cultivation of Plasmodium gonderi.贡氏疟原虫的体外培养
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