Nguyen-Dinh P, Gardner A L, Campbell C C, Skinner J C, Collins W E
Science. 1981 Jun 5;212(4499):1146-8. doi: 10.1126/science.7233207.
The vivax-type simian malaria parasite Plasmodium cynomologi was cultured in vitro by both the candle jar method and the continuous flow technique, with rhesus monkey erythrocytes and RPMI 1640 medium supplemented with Hepes buffer and human serum. After 6 weeks in culture, the growth of the parasite had permitted a 5 X 10(6) cumulative dilution of the original inoculum. Cultured parasites remained infective to rhesus monkeys and exhibited a reversible decrease in the ameboid behavior of their trophozoites.
通过烛缸法和连续流动技术，使用恒河猴红细胞以及添加了Hepes缓冲液和人血清的RPMI 1640培养基，在体外培养间日疟型猴疟原虫食蟹猴疟原虫。培养6周后，疟原虫的生长使得原始接种物累积稀释了5×10⁶倍。培养的疟原虫对恒河猴仍具感染性，并且其滋养体的阿米巴样行为出现了可逆性降低。
