Antoni G, Presentini R, Neri P
Anal Biochem. 1983 Feb 15;129(1):60-3. doi: 10.1016/0003-2697(83)90052-0.
A method for the determination of free amino groups on solid supports is proposed. The method is based on the reaction of the matrix with excess 2,4,6-trinitrobenzenesulfonic acid (TNBS) and subsequent quantitative determination of unreacted TNBS by reaction with glycine. The method is simple and reproducible and does not require the solubilization of the matrix, therefore proving suitable even for crosslinked insoluble supports.
提出了一种测定固体支持物上游离氨基的方法。该方法基于基质与过量的2,4,6-三硝基苯磺酸（TNBS）反应，随后通过与甘氨酸反应对未反应的TNBS进行定量测定。该方法简单且可重复，不需要基质溶解，因此即使对于交联的不溶性支持物也适用。
