Waller R L, Glende E A, Recknagel R O
Biochem Pharmacol. 1983 May 15;32(10):1613-7. doi: 10.1016/0006-2952(83)90336-2.
We have investigated the importance of covalent binding and lipid peroxidation on the depression of microsomal calcium sequestration associated with in vitro metabolism of 14CCl4. Studies with CBrCl3 are also reported. In aerobic systems, promethazine was used to block lipid peroxidation, measured as malondialdehyde (MDA) generation. Effects of low levels of lipid peroxidation were tested in Fe2+-supplemented systems free of halogenated hydrocarbons. The results indicate that microsomal calcium sequestration can be depressed significantly by metabolism of either CCl4 or CBrCl3 in the absence of MDA generation, or by lipid peroxidation occurring in the absence of halogenated hydrocarbons.
我们研究了共价结合和脂质过氧化作用对与四氯化碳体外代谢相关的微粒体钙隔离抑制的重要性。还报告了用三氯溴甲烷进行的研究。在需氧系统中，用异丙嗪来阻断脂质过氧化作用，脂质过氧化作用以丙二醛（MDA）生成量来衡量。在不含卤代烃的亚铁离子补充系统中测试了低水平脂质过氧化作用的影响。结果表明，在没有丙二醛生成的情况下，四氯化碳或三氯溴甲烷的代谢，或者在没有卤代烃的情况下发生的脂质过氧化作用，均可显著抑制微粒体钙隔离。
