Pace-Asciak C R, Rangaraj G
Biochim Biophys Acta. 1983 Feb 7;750(2):330-3. doi: 10.1016/0005-2760(83)90036-x.
Through gas chromatographic techniques with capillary columns and electron-capture detection which allow the resolution of prostaglandins F2 alpha, D2, E2, thromboxane B2 and 6-ketoprostaglandin F1 alpha and their 15-keto-and 15-keto-13,14-dihydro metabolites, we have studied the release of these products from the ductus arteriosus in vitro. 6-Ketoprostaglandin F1 alpha was the major product in the incubation fluid while 15-keto-13,14-dihydro prostaglandin F2 alpha was the major product in the tissue. Prostaglandin E2 was almost undetectable in the fluid and tissue. Prostaglandin I2 formed in major proportions by the tissue is released mostly as 6-ketoprostaglandin F1 alpha, although minor amounts of its 15-keto-13,14-dihydro metabolites were detected. These results show differential release of prostaglandin types by this tissue, demonstrating formation and metabolism of endogenous prostaglandins at the same time.
通过使用毛细管柱和电子捕获检测的气相色谱技术，该技术能够分离前列腺素F2α、D2、E2、血栓素B2和6-酮前列腺素F1α及其15-酮和15-酮-13,14-二氢代谢物，我们在体外研究了这些产物从动脉导管中的释放情况。6-酮前列腺素F1α是孵育液中的主要产物，而15-酮-13,14-二氢前列腺素F2α是组织中的主要产物。前列腺素E2在液体和组织中几乎检测不到。组织中大量形成的前列腺素I2主要以6-酮前列腺素F1α的形式释放，尽管检测到少量其15-酮-13,14-二氢代谢物。这些结果表明该组织对不同类型前列腺素的释放存在差异，同时证明了内源性前列腺素的形成和代谢。
